Abstract:In this experiment,the resistant plant,sensitive plant and the F1 generation were used for identification of the molecular markers linked to salting tolerance gene of Fraxinus velutina by RAPD-BLA.The result indicated that 150 decamer random primers were used to amplify the DNA pools from the resistant plants and sensitive plants,only primer S20 could amplify a 592 bp specific fragment,and named S20-592.SCAR marker was developed from the RAPD marker S20-592 by cloning,sequencing and designing one pair of primers and the SCAR marker only presented in the individuals of the resistant plants.The fragment existed in the resistant plants and F1 population,it was indicated that the SCAR primer can be used for rapid identification of salt tolerance F.velutina species.