To explore the effects of different temperature treatments on anthocyanin contents of postharvest pomegranate pulp,and the function of anthocyanin biosynthesisrelated gene (F3′5′H) in anthocyanin biosynthesis pathway of pomegranate pulp,taking pomegranate cultivar ‘Yushizi’ as test materials,we measured anthocyanin contents of pomegranate pulp under different temperature (0 ℃,5 ℃,10 ℃,15 ℃) treatments.At the same time a anthocyanin biosynthesisrelated gene was cloned from pomegranate pulp by RACE,named PgF3′5′H(GenBank accession:KU058892).The expression levels of PgF3′5′H in pomegranate pulp during different temperature storage periods were analyzed by RTPCR.The results showed that:(1)under different temperature treatments,anthocyanin contents of pomegranate pulp showed a rising trend as the storage time (0-56 d) went on;In the whole storage time,anthocyanin contents were the highest under 15 ℃ treatments,secondly 10 ℃,5 ℃ and 0 ℃ at lower levels;Anthocyanin contents under 15 ℃ were unstable after 14 d.(2)The fulllength cDNA of PgF3′5′H was 1 199 bp with an open reading frame of 918 bp encoding 305 amino acids.The 36th to 39th amino acids of the Nterminus of PgF3′5′H contained the characteristic conserved sequences (CYP motif “PPGP”) of the cytochrome P450 family genes;Bioinformatics showed that the amino acid sequences of PgF3′5′H had high consistency with Eucalyptus grandis EgF3′5′H2,Jatropha curcas JcF3′5′H2 and Nelumbo nucifera NnF3′5′H2 as 86%,82% and 81%,respectively.(3)The expression levels of PgF3′5′H showed a rising trend as the storage time (0-56 d) went on under 0 ℃,5 ℃ and 10 ℃ treatments,being unstable under 15 ℃;The relative expression of PgF3′5′H was significantly positively correlated with anthocyanin contents of pomegranate pulp.The research results laid the foundation for exploring the changes of anthocyanin contents in postharvest pomegranate pulp.