葡萄果实成熟期花色苷与白藜芦醇合成相关基因的表达
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国家自然科学基金地区科学基金(31260456)


Expression of Anthocyanin and Resveratrol Biosynthesis Related Genes in Grapevine Ripening Stage
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    摘要:

    以酿酒葡萄‘赤霞珠’为试材,采用pH示差法和高效液相色谱(HPLC)法分别测定葡萄成熟期果皮花色苷和白藜芦醇含量,用实时荧光定量PCR检测两者合成途径中相关基因的表达量,分析花色苷含量和白藜芦醇含量与其相关基因表达的关系,以揭示结构基因与调控基因的调控机制,为筛选富含花色苷和白藜芦醇的酿酒葡萄提供理论依据。结果显示:(1)葡萄果皮花色苷含量在花后112 d达到最高值(0.77 mg/g),反式白藜芦醇含量在花后126 d达到最高值(30.87 μg/g)。(2)花色苷和白藜芦醇合成途径中,CHSsCHISTSUFGTMybA1、MybA2基因的表达量除花后98 d下调外,其余时间均呈上调表达,而Myb5a则始终呈上调表达。(3)相关分析表明,STS基因表达量与CHS1、CHS2基因表达量呈极显著和显著正相关关系,MybA1、MybA2 基因表达量与CHSsCHISTSUFGT基因的表达量呈极显著正相关关系;Myb5a基因表达量与CHS3基因表达量呈极显著正相关关系。研究表明,部分结构基因的表达与花色苷和白藜芦醇的变化不同步,MybA1和MybA2可能调控花色苷合成途径中多个结构基因的表达,花色苷与白藜芦醇的关系并不固定,而是处在动态变化中。

    Abstract:

    In this study, we used Vitis vinifera L. cv. Cabernet Sauvignon to study anthocyanins and resveratrol accumulation at maturity stage, expression of anthocyanins and resveratrol biosynthesis genes by using HPLC technology, pH differential method and realtime quantitative PCR. The study aims to reveal the regulatory mechanism of structural genes and to provide theoretical basis for screening grape wine rich in anthocyanins and resveratrol. The research findings suggested that: (1) the anthocyanins contents at 112 days after anthesis reached the maximum of 0.77 mg/g, the contents of transresveratrol at 126 days after anthesis reached the maximum of 30.87 μg/g. (2) The expression of CHSs, CHI, STS, UFGT, MybA1 and MybA2 genes in the pathway of anthocyanin and resveratrol synthesis were upregulated, but they were downregulated on 98 days after anthesis. (3) The correlation analysis showed that the expression of STS gene was the most significantly and significantly positively correlated with the expression of CHS1 and CHS2 genes. The expression of MybA1 and MybA2 genes was the most significantly positively correlated with the expression of CHSs, CHI, STS and UFGT genes, and the expression of Myb5a gene was significantly positively correlated with the expression of CHS3 gene. The research showed that the expression of some structural genes was not synchronized with the accumulation of anthocyanins and resveratrol. MybA1 and MybA2 may express multiple structural gene regulation of anthocyanins synthesis pathway. The relationship between anthocyanins and resveratrol was not fixed, but in a dynamic change.

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席 奔,张敏敏,柳巧禛,等.葡萄果实成熟期花色苷与白藜芦醇合成相关基因的表达[J].西北植物学报,2017,37(10):2010-2016

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  • 在线发布日期: 2017-12-01
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