We used unfertilized ovaries of ‘Guiyang xiaoqinggua’ (pumpkin) as the explants to study the heat shock time and TDZ concentration on embryo induction via in vitro culture. Furthermore, we optimized the test steps and researched the method of obtaining regenerated plants directly. The results showed that the best obtained pumpkin plants were inducted regenerated plants directly under dark at 35 ℃ for 5 d, and then moved to light condition at 25 ℃ for 90 d; The unfertilized ovaries can induct regenerated plants in the TDZ concentration at 0.04-0.06 mg/L, the best TDZ concentration was 0.06 mg/L, and the ratio of inducted embryo and regenerated plants were 22.57% and 14.30%, respectively.