茶树GRF基因家族的全基因组鉴定及表达分析
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引用本文:王鹏杰,郑玉成,林 浥,周 珍,杨江帆,叶乃兴.茶树GRF基因家族的全基因组鉴定及表达分析[J].西北植物学报,2019,39(3):413~421
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王鹏杰,郑玉成,林 浥,周 珍,杨江帆,叶乃兴* (福建农林大学 园艺学院/茶学福建省高校重点实验室福州 350002) 
基金项目:福建省“2011协同创新中心”中国乌龙茶产业协同创新中心专项(闽教科[2015]75号);
中文摘要:生长调控因子(growth regulating factor,GRF)是植物特有的转录因子家族,在植物生长发育过程中起重要调控作用。该研究利用生物信息学的方法,从茶树基因组中鉴定获得11个CsGRF转录因子家族成员,且均具有完整的特征结构域QLQ和WRC。CsGRF家族成员含有3~6个外显子,基于系统进化关系将CsGRF家族分为6组,且与葡萄及猕猴桃GRF家族的亲缘关系更为接近。不同组织转录组数据分析结果表明,该家族在生长活跃的茶树嫩梢部位高表达。上游启动子区域分析发现大量与植物发育、激素及胁迫响应密切相关的顺式作用元件。荧光定量检测发现,分别有10个和2个CsGRF成员在低温和干旱胁迫处理后呈现显著上调表达,其中CsGRF8和CsGRF11对2种非生物胁迫均有响应;并发现受ABA、MeJA和GA激素处理诱导分别有9个、3个和6个CsGRF基因的表达水平差异显著。研究表明,CsGRF基因家族在茶树生长发育过程及应激反应中起作用,推测CsGRF基因可能通过各种激素信号转导途径参与胁迫响应过程。
中文关键词:茶树  GRF转录因子  系统进化  胁迫
 
Genome wide Identification and Expression Analysis of GRF Gene Family in Camellia sinensis
Abstract:Growth regulating factor (GRF) is a plant specific transcription factor family that plays an important regulatory role in plant growth and development. In this study, 11 CsGRF transcription factors were identified in tea plant genome with complete characteristic domains QLQ and WRC. The CsGRF family members contained 3 to 6 exons and were divided into 6 groups based on phylogenetic relationships, which are closer to the grape and kiwi. The transcriptome data analysis of different tissues indicated that the GRF family was highly expressed in the tender shoots. Upstream promoter region analysis revealed a large number of cis acting elements closely related to plant development, hormone and stress response. Fluorescence quantitative detection showed that 10 and 2 CsGRF members were up regulated under low temperature and drought stress, respectively, and CsGRF8 and CsGRF11 responded to both abiotic stresses. Furthermore, the expression levels of 9, 3 and 6 CsGRF genes were significantly different by ABA, MeJA and GA hormone treatments, respectively. This work indicates that the CsGRF family genes are involved in the growth and development and stress response in tea plants, speculating that CsGRF genes may play a role in the stress response relying on the hormone signal transduction pathways.
keywords:Camellia sinensis  GRF transcription factors  phylogeny analysis  stress
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