苎麻转录因子基因BnMYB3的克隆及表达分析
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引用本文:朱守晶,史文娟.苎麻转录因子基因BnMYB3的克隆及表达分析[J].西北植物学报,2019,39(3):422~429
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作者单位
朱守晶,史文娟 (宜春学院 生命科学与资源环境学院江西宜春 336000) 
基金项目:江西省教育厅科学技术研究项目(GJJ161008);
中文摘要:该研究采用RACE技术,从苎麻中克隆到1个MYB转录因子基因(BnMYB3)的全长cDNA序列(GenBank登录号为MF741320.1)。生物信息学分析表明,BnMYB3基因cDNA全长为1 216 bp,包括900 bp编码区序列,编码含有299个氨基酸的蛋白,其分子量约为33.63 kD,理论等电点为9.16;该蛋白质含有2个典型的MYB结构域,属于R2R3 MYB。从苎麻基因组中克隆了BnMYB3基因1 681 bp启动子序列,该序列包含ABRE、GARE motif、CGTCA motif和TGACG motif等多个逆境相关的顺式作用元件。实时荧光定量PCR分析表明,BnMYB3为组成型表达基因,在茎和叶中的表达量显著高于根;BnMYB3基因能够响应镉胁迫,且表达量随镉胁迫处理时间和处理浓度的增加而显著上升。
中文关键词:苎麻  MYB转录因子    克隆  表达
 
Cloning and Expression Pattern Analysis of BnMYB3 Transcription Factor Gene in Ramie
Abstract:In this paper, a MYB gene named BnMYB3 was isolated from ramie (Boehmeria nivea L.) by amplification of cDNA ends (RACE), whose GenBank accession number is MF741320.1. The sequence analysis showed that the full length cDNA of BnMYB3 contained 1 216 base pairs, with a coding region of 900 base pairs. This sequence encoded a protein of 299 amino acids in length with a molecular weight of 33.63 kD and a theoretical isoelectric point of 9.16. The protein contains two typical MYB domains which belong to R2R3 MYB gene family. The 1 681 base pairs promoter sequence of BnMYB3 was cloned from the genome of ramie. By bioinformatics analysis, the promoter sequence contained several stress related cis acting elements, such as ABRE, GARE motif, CGTCA motif and TGACG motif. Real time quantitative PCR analysis showed that BnMYB3 was a constitutive gene, but the expression levels of BnMYB3 in stems and leaves were significantly higher than that in roots. Furthermore, BnMYB3 gene was apparently induced by cadmium, and the relative expression level of BnMYB3 increased dramatically with the prolongation of treatment time and the increasement of Cd2+ concentration.
keywords:Boehmeria nivea L.  MYB transcription factor  cadmium  cloning  expression analysis
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