平欧杂种榛叶片解剖结构的抗旱性研究
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引用本文:李嘉诚,罗 达,史彦江,宋锋惠.平欧杂种榛叶片解剖结构的抗旱性研究[J].西北植物学报,2019,39(3):462~471
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李嘉诚1,罗 达2,史彦江2,宋锋惠1,2* (1 新疆农业大学 林学与园艺学院乌鲁木齐 8300522 新疆林业科学院 经济林研究所乌鲁木齐 830063) 
基金项目:新疆维吾尔自治区重点研发项目(2016B01005 2)
中文摘要:为研究平欧杂种榛在新疆干旱地区的生态适应性,以新疆伊犁察布查尔县种质资源汇集圃内的38个品种(系)平欧杂种榛为试验材料,采用石蜡制片法,应用光学显微技术测定了叶片上表皮厚度、下表皮厚度、叶片厚度、主脉厚度、栅栏组织厚度、海绵组织厚度、栅栏组织厚度/海绵组织厚度(栅海比)、栅栏组织结构紧密度和海绵组织结构疏松度等9个抗旱相关的解剖结构特征参数,并进行统计分析和抗旱性综合评价。结果表明:(1)不同品种(系)主脉厚度、上下表皮厚度、叶片厚度、栅栏组织厚度和海绵组织厚度均表现出极显著水平差异,叶片上表皮厚度、下表皮厚度、叶片厚度、栅栏组织厚度和海绵组织厚度之间呈极显著相关和显著相关关系。(2)主成分分析表明,第1、2、3主成分的特征值分别为3.109、2.614和1.884,前3个主成分的累计贡献率达84.52%,基本能概括所有指标的主要信息。(3)采用聚类分析法将38个材料分成5大类;隶属函数法综合评价抗旱能力显示,材料84 48、81 21、84 310、F 03、B 11、85 162的抗旱性较强,材料85 127、85 88、B 21、80 4的抗旱性较差。
中文关键词:平欧杂种榛  叶片解剖结构  聚类分析  隶属函数法  抗旱性
 
Study on Drought Resistance of Leaf Anatomical Structure of Corylus heterophylla×Corylus avellana
Abstract:In order to study the ecological adaptability of Corylus heterophylla×Corylus avellana in the arid regions of Xinjiang, we collected 38 varieties (lines) of C. heterophylla×C. avellana from the planting resources collection in Chabuchaer County, Xinjiang, and applied the paraffin wax method. The optical microscopy technique was used to determine the epidermal thickness, epidermis thickness, leaf thickness, main vein thickness, palisade tissue thickness, sponge tissue thickness, palisade tissue thickness/sponge tissue thickness (gate to sea ratio), palisade tissue tightness and sponge, looseness of tissue structure. These nine drought related anatomical structural parameters were used with statistical analysis and comprehensive evaluation of drought resistance. The results showed that: (1) the differences in main vein thickness, upper and lower epidermis thickness, leaf thickness, palisade tissue thickness and sponge tissue thickness of different varieties (lines) showed extremely significant levels, and there was a significant correlation and significant correlation between epidermal thickness, epidermis thickness, leaf thickness, palisade tissue thickness and sponge tissue thickness. (2) Principal component analysis showed that the eigenvalues of the first, second and third principal components were 3.109, 2.614 and 1.884, respectively. The cumulative contribution rate of the first three principal components was 84.52%, which basically summarized the main information of all indicators. (3) The cluster analysis method was used to divide 38 varieties (lines) into five categories. The comprehensive evaluation of drought resistance by the membership function method showed that 84 48, 81 21, 84 310, F 03, B 11, and 85 162 had strong drought resistance, 85 127, 85 88, B 21, and 80 4 drought resistance is poor.
keywords:Corylus heterophylla×Corylus avellana  leaf anatomical structure  cluster analysis  membership function method  drought resistance
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