百合育性与花药发育过程中激素和内源物质含量及能量代谢酶活性的关系
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引用本文:贾文杰,郑 洁,崔光芬,马璐琳,段 青,杜文文,王祥宁.百合育性与花药发育过程中激素和内源物质含量及能量代谢酶活性的关系[J].西北植物学报,2019,39(3):480~488
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贾文杰,郑 洁,崔光芬,马璐琳,段 青,杜文文,王祥宁* (国家观赏园艺工程技术研究中心云南省农业科学院花卉研究所云南省花卉育种重点实验室昆明 650205) 
基金项目:国家自然科学基金(31360489);
中文摘要:为揭示百合雄性不育发生与其花药内源激素含量、几种内源物质含量和能量代谢酶活性变化的关系,该研究利用高效液相色谱法、串联质谱法和比色法,测定了可育系和不育系花药发育关键时期的激素、可溶性糖、可溶性蛋白、丙二醛、脯氨酸含量和COD及ATP能量代谢酶活性,分析激素含量、各激素间比值、内源物质含量和能量代谢酶活性对百合雄性不育的影响。结果表明:(1) 在百合花药造孢细胞期到四分体期,花药内源激素含量、内源物质含量及能量代谢酶活性变化在不育系与可育系间均存在差异。其中,不育系花药的生长素(IAA) 含量在发育的各阶段均高于可育系;ZR和GA4含量在花粉母细胞期不育系与可育系相差不大,但到四分体期不育系中的含量低于可育系;ABA含量在不育系四分体期出现盈积;JA含量在不育系的花粉母细胞期低于可育系,而在四分体期高于可育系。(2)花药内源物质中可溶性糖、可溶性蛋白和脯氨酸含量表现为可育系均高于不育系,但不育系的丙二醛含量在四分体期显著高于可育系。(3)COD和ATP能量代谢酶活性在花药发育的各阶段均是可育系高于不育系。研究认为,在百合不育系花药发育关键时期(花粉母细胞期到四分体期),其IAA含量增加,ABA水平提高,GA4和ZR含量降低,影响了花药内源物质和能量的正常代谢,这可能是导致百合花药中花粉发育受阻、形成败育的原因。
中文关键词:百合  雄性不育  植物激素  内源物质  能量代谢酶活性
 
Relationship between Lilium Fertility and Hormone, Endogenous Substance Content, Energy Metabolism Enzyme Activity during Anther Development
Abstract:In order to reveal the relationship between Lilium male sterile and fertile lines in changes of hormones, some endogenous substances contents and energy metabolism enzyme activities, this study used chromatography tandemmass spectrometry and high performance liquid chromatography to test hormones. Endogenous substances and energy metabolism enzyme activities were measured by colorimetry. The effects of hormone content, hormone ratio, endogenous substance content and energy metabolism enzyme activity on male sterility of Lilium were analyzed. The results show that: (1) during the development period which is from sporogenous cell stage to tetrad stage, changes of endogenous hormone, endogenous substance content and energy metabolic enzyme activity in male sterile line and fertile line were different. The content of IAA in male sterile line was higher than that in fertile line at all stages. The contents of ZR and GA4 in male sterile and fertile line were similar at pollen mother cell stage, but two contents in male sterile line were lower than those in fertile line at tetrad stage. ABA content in male sterile line accumulated at tetrad stage. JA content in male sterile line was lower than it in the fertile line at the pollen mother cell stage. However, the trend is opposite at the tetrad stage. (2)The contents of soluble sugar, soluble protein and proline in fertile line were higher than those in male sterile line at all stages. However, the content of MDA in male sterile line was significantly higher than that in fertile line at tetrad stage. (3)The activities of COD and ATP in fertile line were higher than those in sterile line at all anther development stages. This study suggest that: from sporogenous cell stage to tetrad stage, high IAA, ABA and low GA4, ZR contents may lead to the deficiency of soluble sugar, soluble protein, proline contents and lower energy metabolism enzyme activities. These are the possible reasons to cause male sterile phenomenon of Lilium.
keywords:Lilium  male sterile  hormone  endogenous substances  energy metabolism enzyme activities
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