濒危羊踯躅子代幼苗遗传多样性的SSR分析
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引用本文:董丽敏,戴亮芳,白李唯丹,刘梦露,谢建坤,罗向东.濒危羊踯躅子代幼苗遗传多样性的SSR分析[J].西北植物学报,2019,39(4):613~619
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作者单位
董丽敏,戴亮芳,白李唯丹,刘梦露,谢建坤,罗向东* (江西师范大学 生命科学学院南昌 330022) 
基金项目:国家自然科学基金(31360147,31660384);
中文摘要:利用本课题组此前开发的20对多态性较高的羊踯躅SSR引物,分析来自3个省份的4个羊踯躅野生幼苗自然居群(共64个株系)的遗传多样性和遗传结构,以期为羊踯躅保护提供科学依据。结果显示:(1)20对SSR引物共扩增出314个等位基因,每个SSR位点的平均等位基因数为15.700,多态信息含量PIC和有效等位基因数Ne的均值分别为0.850和4.457。(2)羊踯躅幼苗自然居群的基因多样性指数H和Shannon多样性指数I的均值分别为0.717和1.557,其中江西金溪(JX)居群的遗传多样性最丰富,浙江磐安(PA)的最低。(3)基于无限等位基因模型(IAM)分析发现,羊踯躅幼苗种群的基因流Nm和遗传分化系数Fst分别为1.372和0.155;AMOVA分析表明,羊踯躅幼苗种群的86.0%变异发生于居群内,仅有14%变异发生在居群间。(4)遗传距离法聚类NJ分析和Structure分析结果基本一致,分别聚为3大类和4大类。综合比较分析发现,羊踯躅幼苗种群中的各个遗传多样性相关的指标参数均低于成年自然居群,表明生境片段化已对羊踯躅的生存构成了极大的威胁。
中文关键词:野生羊踯躅幼苗  SSR  遗传结构  濒危植物
 
Genetic Diversity of Wild Seedlings of Endangered Rhododendron molle G. Don by SSR
Abstract:The genetic diversity and genetic structure of 4 wild seedling populations of Rhododendron molle (64 individuals) from 3 provinces were analyzed with 20 pairs of polymorphic simple sequence repeat (SSR) primers developed by our group. We aim to provide some theoretical basis for the conservation of R. molle. The results showed: (1) 314 alleles were amplified by 20 pairs of SSR primers, the average number of alleles of each SSR site was 15.700, and the mean values of polymorphic information content (PIC) and the effective alleles number (Ne) were 0.850 and 4.457, respectively. (2) The mean value of the genetic diversity index (H) and Shannon diversity index (I) of the seedling population of R. molle were 0.717 and 1.557, respectively. The genetic diversity of Jinxi (Jiangxi Province) population exhibits great level of variability, whereas the population of Panan (Zhejiang Province) exhibits the lowest level of variability. (3) Based on the analysis of the infinite allele model (IAM), it was found that the gene flow (Nm) and the genetic differentiation coefficient (Fst) of the seedling population of R. molle (Fst) were 1.372 and 0.155, respectively. AMOVA analysis showed that the probability of variation of the seedling population of R. molle mainly occurred in the middle population (86.0%), and the inter population variation was only 14%; (4) The results of neighbor joining (NJ) analysis and structure classification were basically similar, which are divided into 3 and 4 groups, respectively. Comprehensive comparative analysis found that the index parameters related to the genetic diversity of the seedling population of R. molle were all lower than the natural population of adults, which indicated that the fragmentation of habitat had posed a great threat to the survival of R. molle.
keywords:wild seedlings of R. molle  SSR  genetic structure  endangered plants
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