温度和盐胁迫对草莓开花进程和相关基因表达的影响
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引用本文:董天宇,朱旭东,张克坤,房经贵,石倩蔚,贾海锋.温度和盐胁迫对草莓开花进程和相关基因表达的影响[J].西北植物学报,2019,39(4):638~647
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董天宇,朱旭东,张克坤,房经贵,石倩蔚,贾海锋* (南京农业大学 园艺学院南京 210095) 
基金项目:江苏省优秀青年基金(BK20180076);
中文摘要:为探究草莓花器官对高温、低温、盐胁迫的响应特征,该研究以草莓品种‘甜查理’为试材,设置0 ℃、4 ℃、25 ℃(CK)、37 ℃、200 mmol/L NaCl、400 mmol/L NaCl等逆境胁迫处理,考察草莓花外观形态、生长发育状态、花器官抗氧化酶活性, 以及3个草莓花器官分生组织特异性基因、12个成花途径基因、3个抗氧化酶基因、6个抗逆基因的表达等。结果显示:(1)0 ℃、4 ℃、37 ℃、200 mmol/L NaCl、400 mmol/L NaCl溶液处理均抑制了草莓花的开放,0 ℃低温显著抑制草莓花器官的开放,并对草莓的花器官造成伤害。(2)当草莓植株受到高温、低温、盐胁迫时,其花器官内抗氧化酶SOD、POD、CAT活性及MDA含量均得到提高,其相关抗氧化酶基因的表达水平也相应提高。(3)0 ℃、4 ℃、37 ℃以及200 mmol/L NaCl、400 mmol/L NaCl溶液处理均显著降低了草莓花器官FaAP1、FaLFYFaFDFaSOC1等成花基因的表达量;0 ℃低温诱导了FaICEFaRD29AFaCORFaAFP 等4个抗寒基因的表达水平;200 mmol/L NaCl、400 mmol/L NaCl的盐胁迫显著提高了FaP5CS基因的表达量。研究表明,0 ℃、4 ℃、37 ℃、200 mmol/L NaCl、400 mmol/L NaCl溶液处理不同程度抑制了草莓的开花进程,对草莓花器官造成伤害;不同逆境胁迫处理显著提高了草莓花器官SOD、POD、CAT活性以及MDA含量,相应的抗氧化酶基因表达得到上调,同时显著降低了草莓成花途径相关基因表达,增强了相关抗逆基因的表达水平。
中文关键词:草莓  开花进程  逆境胁迫  酶活性  基因表达
 
Effect of Temperature and Salt Stress on the Flowering Process of Strawberry
Abstract:In order to explore the response characteristics of strawberry flower organs to high temperature, low temperature and salt stress, we used strawberry variety ‘Sweet Charlie’ as the test material. 0 ℃, 4 ℃, 25 ℃ (CK), 37 ℃, 200 mmol/L NaCl, 400 mmol/L NaCl and other stress treatments were used to investigate the appearance, growth and development of flowering organs, antioxidant enzyme activities of flower organs, and the expression of three strawberry flower organ meristem genes, 12 genes of flowering pathways, and three antioxidants enzyme genes and six stress resistant genes. The results showed that: (1) 0 ℃, 4 ℃, 37 ℃, 200 mmol/L NaCl, 400 mmol/L NaCl treatment inhibited the opening of strawberry flowers, 0 ℃ low temperature significantly inhibited the opening of strawberry flower organs, and caused the flower organs of strawberries hurt. (2) When strawberry plants were exposed to high temperature, low temperature and salt stress, the activities of antioxidant enzymes SOD, POD, CAT and MDA content in flower organs were increased, and the expression levels of related antioxidant enzyme genes were also increased. (3) 0 ℃, 4 ℃, 37 ℃ and 200 mmol/L NaCl, 400 mmol/L NaCl solution significantly reduced the expression of FaAP1, FaLFY, FaFD, FaSOC1 and other flowering genes in strawberry flower organs; 0 ℃ low temperature induced the expression levels of four cold resistant genes such as FaICE, FaRD29A, FaCOR and FaAFP; salt stress of 200 mmol/L NaCl and 400 mmol/L NaCl significantly increased the expression of FaP5CS gene. Studies showed that 0 ℃, 4 ℃, 37 ℃, 200 mmol/L NaCl, 400 mmol/L NaCl solution inhibited the flowering process of strawberry and caused damage to strawberry flower organs. Different stress treatment significantly increased the SOD of strawberry flower organs. The activities of POD, CAT and MDA content, the expression of the corresponding antioxidant enzyme genes were up regulated, and the expression of genes involved in the flowering pathway of strawberry was significantly decreased, and the expression level of related stress resistant genes was enhanced.
keywords:strawberry  flowering process  adversity stress  enzyme activity  gene expression
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