In order to explore the response characteristics of strawberry flower organs to high temperature, low temperature and salt stress, we used strawberry variety ‘Sweet Charlie’ as the test material. 0 ℃, 4 ℃, 25 ℃ (CK), 37 ℃, 200 mmol/L NaCl, 400 mmol/L NaCl and other stress treatments were used to investigate the appearance, growth and development of flowering organs, antioxidant enzyme activities of flower organs, and the expression of three strawberry flower organ meristem genes, 12 genes of flowering pathways, and three antioxidants enzyme genes and six stressresistant genes. The results showed that: (1) 0 ℃, 4 ℃, 37 ℃, 200 mmol/L NaCl, 400 mmol/L NaCl treatment inhibited the opening of strawberry flowers, 0 ℃ low temperature significantly inhibited the opening of strawberry flower organs, and caused the flower organs of strawberries hurt. (2) When strawberry plants were exposed to high temperature, low temperature and salt stress, the activities of antioxidant enzymes SOD, POD, CAT and MDA content in flower organs were increased, and the expression levels of related antioxidant enzyme genes were also increased. (3) 0 ℃, 4 ℃, 37 ℃ and 200 mmol/L NaCl, 400 mmol/L NaCl solution significantly reduced the expression of FaAP1, FaLFY, FaFD, FaSOC1 and other flowering genes in strawberry flower organs; 0 ℃ low temperature induced the expression levels of four coldresistant genes such as FaICE, FaRD29A, FaCOR and FaAFP; salt stress of 200 mmol/L NaCl and 400 mmol/L NaCl significantly increased the expression of FaP5CS gene. Studies showed that 0 ℃, 4 ℃, 37 ℃, 200 mmol/L NaCl, 400 mmol/L NaCl solution inhibited the flowering process of strawberry and caused damage to strawberry flower organs. Different stress treatment significantly increased the SOD of strawberry flower organs. The activities of POD, CAT and MDA content, the expression of the corresponding antioxidant enzyme genes were upregulated, and the expression of genes involved in the flowering pathway of strawberry was significantly decreased, and the expression level of related stressresistant genes was enhanced.