Abstract:This study was conducted to explore the expression of the genes associated with anthocyanins biosynthesis and the differences of anthocyanins accumulation in the peel and pulp of one new redflesh grape, ‘Zhongshanhongyu’ (ZSHY). Highperformance liquid chromatography with mass spectrometry (HPLC/MS) and qRTPCR were used to understand the laws of anthocyanins accumulation and related genes expression during the berry development. It aims to provide a theoretical basis for improving quality of grapes. The results showed that: (1) there are total 13 kinds of anthocyanins were detected from the peel and pulp of ‘Zhongshanhongyu’ and all of the anthocyanins were monoglucoside or monogalactoside. It is suggested that ‘ZSHY’ probably belong to the cultivar Vitis vinifera. (2) One of detected anthocyanis, Pelargonidin3Oglucoside barely contained in the cultivar V. vinifera was detected in the peel and pulp of our material. Besides, the composition and content of anthocyanins was also various in different tissues. In the peel of ‘Zhongshanhongyu’, the content of Delphinidinbased anthocyanins was significantly higher than Cyanidinbased anthocyanins, while the contents of Delphinidinbased and Cyanidinbased anthocyanins were almost same with each other. This result may due to the higher expression of F3′5′H in the peel of ‘Zhongshanhongyu’. In addition, the ratio of acylation anthocyanins was higher in the peel than in the pulp. (3) The regulation of the expression level of MYBA2 was consistent with UFGT, and UFGT barely expressed in the pulp, while MYBA2 maybe the key point that could regulate the pulp veraison. Besides, the expression of PYL1 which related to the regulation of ABA, also in according with the expression of OMT and LDOX.