红肉葡萄果实发育过程中花青苷组分变化与基因表达规律分析
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引用本文:王倩兰,李 慧,郑 焕,陶建敏.红肉葡萄果实发育过程中花青苷组分变化与基因表达规律分析[J].西北植物学报,2019,39(4):648~659
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作者单位
王倩兰,李 慧,郑 焕,陶建敏* (南京农业大学 园艺学院南京 210095) 
基金项目:国家现代农业葡萄产业技术体系(CARS 29);
中文摘要:以红肉葡萄新种质‘钟山红玉’为实验材料,通过高效液相色谱 质谱联用(HPLC MS)检测‘钟山红玉’从幼果到成熟期果皮、果肉中的花青苷组分及含量,并利用实时荧光定量PCR检测不同发育时期花青苷合成相关基因的表达水平,研究其不同果实发育时期果皮、果肉中的花青苷组分变化,以及相关基因表达规律,探索红肉葡萄果实呈色机制,为葡萄果实品质改良育种提供理论依据。结果表明:(1)‘钟山红玉’果皮和果肉中共检测出13种花青苷且都为单糖苷花青苷,与欧亚种葡萄亲缘关系较近。(2)‘钟山红玉’果皮、果肉中均检测出了欧亚种葡萄中很少有的天竺葵素3 O 葡萄糖苷;果皮中飞燕草素类(飞燕草素 、锦葵色素 、矮牵牛素 )花青苷衍生物含量显著高于矢车菊素类(矢车菊素 、芍药素 )花青苷衍生物,而在果肉中则两大类花青苷含量相近,这与果皮中较高的F3′5′H基因表达量有关,且在果皮中酰基化花青苷比例显著高于果肉。(3)花青苷合成相关基因MYBA2和UFGT在‘钟山红玉’不同发育时期的表达变化规律一致,UFGT基因在果肉中基本不表达,而MYBA2可能是决定‘钟山红玉’果肉转色的关键因子;并且ABA响应相关基因PYL1在‘钟山红玉’发育时期的表达规律与OMTLDOX基因一致。
中文关键词:红肉葡萄  花青苷  基因表达  高效液相色谱 质谱联用(HPLC MS)
 
Analysis of Anthocyanin Compositons and Gene Expression in Red flesh Grape during the Berry Development
Abstract:This study was conducted to explore the expression of the genes associated with anthocyanins biosynthesis and the differences of anthocyanins accumulation in the peel and pulp of one new red flesh grape, ‘Zhongshanhongyu’ (ZSHY). High performance liquid chromatography with mass spectrometry (HPLC/MS) and qRT PCR were used to understand the laws of anthocyanins accumulation and related genes expression during the berry development. It aims to provide a theoretical basis for improving quality of grapes. The results showed that: (1) there are total 13 kinds of anthocyanins were detected from the peel and pulp of ‘Zhongshanhongyu’ and all of the anthocyanins were monoglucoside or monogalactoside. It is suggested that ‘ZSHY’ probably belong to the cultivar Vitis vinifera. (2) One of detected anthocyanis, Pelargonidin 3 O glucoside barely contained in the cultivar V. vinifera was detected in the peel and pulp of our material. Besides, the composition and content of anthocyanins was also various in different tissues. In the peel of ‘Zhongshanhongyu’, the content of Delphinidin based anthocyanins was significantly higher than Cyanidin based anthocyanins, while the contents of Delphinidin based and Cyanidin based anthocyanins were almost same with each other. This result may due to the higher expression of F3′5′H in the peel of ‘Zhongshanhongyu’. In addition, the ratio of acylation anthocyanins was higher in the peel than in the pulp. (3) The regulation of the expression level of MYBA2 was consistent with UFGT, and UFGT barely expressed in the pulp, while MYBA2 maybe the key point that could regulate the pulp veraison. Besides, the expression of PYL1 which related to the regulation of ABA, also in according with the expression of OMT and LDOX.
keywords:red flesh grape  anthocyanin  gene expression  high performance liquid chromatography with mass spectrometry (HPLC MS)
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