铁核桃叶片多酚含量与相关酶活性及抗氧化能力的关系
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引用本文:张 睿,史斌斌,张文娥,李 雪,潘学军.铁核桃叶片多酚含量与相关酶活性及抗氧化能力的关系[J].西北植物学报,2019,39(4):677~684
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张 睿,史斌斌,张文娥,李 雪,潘学军* (贵州大学贵州省果树工程技术研究中心贵阳 550025) 
基金项目:贵州省高层次创新型人才培养项目(黔科合人才[2016]4038号);
中文摘要:以铁核桃‘黔核7号’叶片为试材,研究了叶片发育过程中多酚含量、多酚代谢相关酶活性、抗氧化能力变化以及它们之间的调控关系。结果表明:(1)‘黔核7号’叶片中酚酸和黄酮类物质是主要的抗氧化多酚;DAPHS、PAL、C4H和4CL 等4种酶在多酚合成中发挥着重要作用;而叶片发育后期多酚分解代谢相关酶(POD,PPO)活性较高,且PPO在多酚氧化中起主要作用。(2)叶片发育进程中,叶片多酚提取液对ABTS及DPPH自由基清除能力总体表现为降低趋势,而对FRAP和铁氰化钾还原能力(PFRAP)则表现为上升趋势。(3)叶片发育进程中,其酚酸含量与PAL和C4H活性呈显著正相关关系,与DAPHS活性的正相关性也较高;PPO活性与酚酸及黄酮类化合物含量呈显著负相关关系。(4)叶片酚酸含量与4种抗氧化能力指标的相关性均达到显著或极显著水平,合成酶中的PAL、C4H和CHI活性与抗氧化能力指标之间的相关性也强于其他酶,其中与ABTS及DPPH自由基清除能力呈正相关关系,与FRAP和PFRAP呈负相关关系;而氧化酶PPO和POD活性与ABTS及DPPH自由基清除能力呈负相关关系,与FRAP和PFRAP呈正相关关系。
中文关键词:核桃叶片  多酚  多酚代谢酶  抗氧化活性  相关性
 
Relationship between Polyphenol Content and Enzymes Activities and Antioxidant Capacity in Juglans sigillata Dode Leaves
Abstract:The relations among phenolics content, activities of enzymes related to phenolics metabolization and antioxidant activity in leaves of Juglans sigillata Dode. cv.‘Qianhe 7’were explored at different development periods. The results showed: (1) the phenolic acids and flavonoids were the most content compounds of phenolics, and four kinds of enzymes, namely DAPHS, PAL, C4H and 4CL play the important roles in the phenolics biosynthesis. While POD and PPO related decomposition were higher at leaf mature period, and the PPO is responsible for polyphenol oxidation. (2) With the development of leaves, the total polyphenol extract of the leaves showed a decreasing trend on the ABTS and DPPH free radical, while the reduction capacity of PFRAP and PFRAP increased continuously, reaching a peak in early August, and then remained at a high level. (3) With the development of leaves, phenolic acids were positively correlated to activities of PAL, C4H, the content of phenolic acids and flavonoids were strongly correlated to activities of DAPHS. Furthermore, the total contents of phenolic acids and flavonoids were negatively correlated to PPO. (4) The phenolic acids content was significant or extremely significant correlated to four kinds of antioxidant activity, the correlations between PAL, C4H, CHI and antioxidant activity were stronger than those of others EAB. The activities of PAL, C4H and CHI were positively correlated to IC50 values of DPPH and ABTS+, and negatively correlated to values of FRAP and PFRAP. While the activities of PPO and POD were negatively correlated to IC50 values of DPPH and ABTS+, and positively correlated to values of FRAP and PFRAP.
keywords:walnut leaf  polyphenol  enzymes related to polyphenol metabolization  antioxidant activity  correlation
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