日本结缕草转录因子基因ZjDREB1.4的功能特征分析
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引用本文:冯婉倩,李 京,邹慧敏,王晓宇,张 红,韦善君.日本结缕草转录因子基因ZjDREB1.4的功能特征分析[J].西北植物学报,2019,39(5):848~856
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冯婉倩,李 京,邹慧敏,王晓宇,张 红,韦善君* (中央民族大学 生命与环境科学学院北京 100081) 
基金项目:国家自然科学基金(31100570);
中文摘要:DREB1(dehydration responsive element binding protein1)是植物中广泛存在的转录因子,在应答非生物胁迫中起重要作用。该研究根据转录组测序数据,从暖季型植物日本结缕草‘Meyer’(Zoysia japonica Steud.)中克隆得到1个DREB1类转录因子基因,命名为ZjDREB1.4。结果表明:(1)该基因推测编码226个氨基酸,含有一个AP2结构域,其上下游具有DREB1组的典型特征序列。(2)半定量RT PCR检测显示,在日本结缕草叶组织中,ZjDREB1.4基因在4 ℃低温、高盐和干旱胁迫下均上调表达,其中受低温诱导上调最显著。(3)亚细胞定位分析显示,ZjDREB1.4 GFP融合蛋白主要定位在细胞核中。(4)酵母单杂交结果显示,ZjDREB1.4蛋白具有强的转录激活功能,但与以ACCGAC为核心序列的DRE元件结合功能较弱。(5)与野生型拟南芥相比,超表达ZjDREB1.4的拟南芥植株的营养生长无明显改变,仅抽薹时间推迟3~8 d,但是对高温和冷冻低温胁迫的耐受能力明显增强。ZjDREB1.4基因具有抗逆功能,对植株生长发育的负效应又小,其在植物育种中的应用潜力值得进一步探究。
中文关键词:日本结缕草  非生物胁迫  DREB转录因子  DRE结合活性  生理功能
 
Characteristics of a Transcription Factor Gene ZjDREB1.4 from Zoysia japonica
Abstract:DREB1 (dehydration responsive element binding protein 1) belongs to a subfamily of transcription factors found in plants that plays a key role during the response to abiotic stresses. On the basis of transcriptome sequencing data, we report a DREB1 gene, designated ZjDREB1.4 in this study, that was isolated from zoysiagrass (Zoysia japonica Steud.), a popular warm season turfgrass. (1) The ZjDREB1.4 gene encodes a protein that contains 226 amino acids and has an AP2 domain flanked by sequences characteristic of DREB1 proteins from other species. (2) The expression of ZjDREB1.4 was significantly induced by 4 ℃ cold stress, moderately induced by high salt and slightly induced by drought stress. (3) The product of the fusion gene ZjDREB1.4 GFP was primarily targeted to the nucleus. (4) Yeast one hybrid assays indicated that ZjDREB1.4 showed strong transactivation activity but weak binding to the DRE in which ACCGAC was the core sequence. (5) Transgenic Arabidopsis plants overexpressing ZjDREB1.4 showed similar growth to WT (wild type) with only a 3-8 day delay in bolting, but showed higher survival rates with high temperature or freezing temperature stress. Since ZjDREB1.4 functions in stress tolerance with weak adverse effect on plant growth and development, the potential usage of it in plant breeding is worthy of further study.
keywords:Zoysia japonica  abiotic stress  transcription factor DREB  DRE binding activity  physiological function
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