茶树BES1转录因子全基因组鉴定与分析
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引用本文:陈雪津,王鹏杰,郑玉成,陈 笛,郭永春,叶乃兴.茶树BES1转录因子全基因组鉴定与分析[J].西北植物学报,2019,39(5):876~885
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陈雪津,王鹏杰,郑玉成,陈 笛,郭永春,叶乃兴* (福建农林大学 园艺学院,茶学福建省高校重点实验室福州 350002) 
基金项目:福建省“2011协同创新中心”中国乌龙茶产业协同创新中心专项(闽教科[2015]75号);
中文摘要:植物特异性转录因子 (BRI1 EMS SUPPRESSOR1,BES1)家族参与调节油菜素内酯(brassinosteroids,BR)信号通路,在植物生长和应对非生物胁迫的反应中发挥重要作用。该研究利用生物信息学方法对茶树BES1转录因子家族进行鉴定,分析茶树CsBES1基因在8个茶树组织中的表达模式,并对CsBES1转录因子家族在低温、干旱及ABA激素处理下的表达进行分析,以揭示茶树CsBES1转录因子家族的功能及其对环境胁迫的响应。结果显示:(1)从茶树全基因组中鉴定获得10个茶树BES1转录因子家族成员,均具有完整的BES1_N结构域;根据系统进化关系将10个CsBES1转录因子家族分成3组,分别包含2个、3个和5个成员;该家族成员每个基因含有2~11个外显子。(2)组织表达模式分析显示,茶树BES1转录因子家族在生长活跃的茶树嫩梢和成熟叶中表达量较高,不同成员之间表达存在差异性。(3)上游启动子区域分析发现大量与植物激素和非生物胁迫响应相关的顺式作用元件。(4)荧光定量检测发现,BES1基因在茶树不同胁迫处理下的表达模式不同,在低温和脱落酸处理下,CsBES1 1、CsBES1 7、CsBES1 8和CsBES1 9基因的表达量显著提高,而在干旱处理下,CsBES1 2、CsBES1 4、CsBES1 5、CsBES1 6、CsBES1 7、CsBES1 8和CsBES1 9基因的表达量显著提高。
中文关键词:茶树  BES1转录因子  系统进化  非生物胁迫  表达分析
 
Genome wide Identification and Analysis of BES1 Transcription Factor Family in Camellia sinensis
Abstract:Plant specific transcription factor (BRI1 EMS SUPPRESSOR1,BES1) family involved in regulating the brassinosteroids (BR) signaling pathway and play an important role in plant growth and response to abiotic stress. In this study, the BES1 transcription factor family of tea plant was identified by bioinformatics method. The expression patterns of tea CsBES1 gene in 8 tea tissues were analyzed, and the CsBES1 transcription factor family was expressed at low temperature. In order to reveal the function of CsBES1 transcription factor family and its response to environmental stress, we analyzed the expression of CsBES1 transcription factor family in tea plants under drought and ABA hormone treatment. The results showed that: (1) ten members of the BES1 transcription factor family of tea tree were identified from the whole genome of tea tree, all of which had complete BES1_N domain. According to phylogenetic relationships, 10 CsBES1 transcription factor families were divided into 3 groups, including 2, 3 and 5 members respectively. Each gene of this family contains 2~11 exons. (2) The analysis of tissue expression pattern showed that the expression of BES1 transcription factor family was higher in the young shoots and mature leaves of tea plants, and there were differences among different members. (3) The upstream promoter region analysis showed that there was a lot of cis elements associated with various stress and hormone responses. (4) The results of fluorescence quantitative analysis showed that the expression patterns of BES1 gene were different under different stress conditions. Under low temperature and abscisic acid treatment, the expression levels of CsBES1 1, CsBES1 7, CsBES1 8 and CsBES1 9 genes increased significantly. However, the expression of CsBES1 2, CsBES1 4, CsBES1 5, CsBES1 6, CsBES1 7, CsBES1 8 and CsBES1 9 genes increased significantly under drought stress.
keywords:Camellia sinensis  BES1 transcription factors  phylogeny analysis  abiotic stress  expression analysis
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