小麦TaTrxh9基因的序列特征及其对渗透胁迫的响应
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引用本文:李梦园,樊亚栋,张新宁,姜玉梅,孟凡荣,李永春.小麦TaTrxh9基因的序列特征及其对渗透胁迫的响应[J].西北植物学报,2019,39(5):896~903
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李梦园1,樊亚栋2,张新宁1,姜玉梅2,孟凡荣1,李永春2* (1 河南农业大学 生命科学学院 郑州 4500022 河南农业大学 农学院 郑州 450046) 
基金项目:国家自然科学基金(31571655);
中文摘要:硫氧还蛋白是一类广泛存在于植物中的低分子量氧化还原蛋白,在植物生长发育调控和逆境响应过程中发挥重要的调控功能。以小麦(Triticum aestivum L.)品种‘中国春’和‘洛旱2号’为材料,采用RT RCR方法克隆了小麦硫氧还蛋白基因TaTrxh9,通过生物信息学方法分析了其染色体定位及序列特征,并利用qRT PCR技术分析了该基因对渗透胁迫的响应模式。结果显示:(1)成功克隆到小麦TaTrxh9基因,序列分析发现TaTrxh9基因位于第1染色体群的短臂上,并将其3个部分同源基因命名为TaTrxh9 1ASTaTrxh9 1BSTaTrxh9 1DS。(2)TaTrxh9基因包含4个内含子,且在内含子区域3个部分同源基因间序列差异较大;3个部分同源基因的编码区序列一致性在98%以上,均编码131个氨基酸,且蛋白序列完全相同;TaTrxh9蛋白包含1个Trx family保守域和CXXC氧化还原活性位点,三维结构预测分析显示该蛋白可形成典型的硫氧还蛋白空间结构,包括由4个α螺旋组成的外围框架和由5个反向平行的β折叠组成的中心轴。(3)qRT PCR分析显示,TaTrxh9基因受干旱胁迫诱导在叶片中下调表达,而在根系中呈先上调后下调的表达模式;盐胁迫过程中,TaTrxh9基因在叶片中无显著响应,在根系中也仅出现短暂的诱导表达现象;在ABA处理过程中,TaTrxh9基因表达模式与其对干旱胁迫的响应模式类似。研究推测,TaTrxh9基因对干旱胁迫的响应过程可能与ABA介导的基因表达调控有关。
中文关键词:小麦  硫氧还蛋白  基因克隆  渗透胁迫  表达模式
 
Sequence Characteristics of TaTrxh9 Gene and Its Response to Osmotic Stresses in Wheat
Abstract:Thioredoxins are a kind of low molecular weight redox proteins widely existing in plants, which play important roles in the developmental regulation and stress responding processes in plants. Two wheat (Triticum aestivum L.) cultivars, ‘Chinese Spring’ and ‘Luohan No. 2’, were used in this study. A thioredoxin gene TaTrxh9 was cloned from common wheat by using RT PCR. Bioinformatics analysis was performed to define the chromosomal localization and sequence characteristics of TaTrxh9, and its expression patterns responding to osmotic stresses were detected by qRT PCR. The results showed that: (1) TaTrxh9 gene was successfully cloned, sequence analysis indicated that TaTrxh9 was located on the short arms of group one chromosomes, and three homoeologues of the gene were designated as TaTrxh9 1AS, TaTrxh9 1BS and TaTrxh9 1DS, respectively. (2) Four introns were detected in each of TaTrxh9 homoeologues and a large number of polymorphisms were detected in introns among homoeologues, while the coding regions of homoeologues were highly identical (>98%) and encoded an identical protein sequence with the length of 131 amino acids. Characterization of TaTrxh9 protein indicated that a TRX family conserved domain and a CXXC redox active site was detected, and the TaTrxh9 was predicted to have a typical spatial structure of thioredoxin, including a peripheral framework composed of 4 helixes and a central axis composed of 5 antiparallel β sheets. (3) qRT PCR analysis showed that TaTrxh9 gene was down regulated in leaves under drought stress, while initially increased and then declined in roots. Under salt stress, no significant response of TaTrxh9 gene was detected in leaves and only a transient induction was observed in roots. The expression pattern of TaTrxh9 under treatment of ABA is similar to that under the drought stress. It was speculated that the responding process of TaTrxh9 gene to drought stress was associated with ABA mediated regulation of gene expression.
keywords:wheat  thioredoxin  gene cloning  osmotic stress  expression pattern
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