棉花肉桂醇脱氢酶基因在棉纤维中的表达及对其结构组分的影响
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引用本文:胡文冉,李晓东,周小云,杨 洋,李 波,李晓荣.棉花肉桂醇脱氢酶基因在棉纤维中的表达及对其结构组分的影响[J].西北植物学报,2019,39(6):1114~1120
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胡文冉1,李晓东2,周小云1,杨 洋1,李 波1,李晓荣1* (1 新疆农业科学院 核技术生物技术研究所新疆农作物生物技术重点实验室 乌鲁木齐8300912 新疆维吾尔自治区水产科学研究所 乌鲁木齐830000) 
基金项目:新疆维吾尔自治区重点实验室项目(2016D03013)
中文摘要:利用农杆菌介导法获得‘新陆早36号’转棉花肉桂醇脱氢酶基因(GhCAD6)材料,以转基因T6代植株为试材,对GhCAD6基因在叶片基因组中的整合情况和不同发育阶段棉花纤维中的表达量进行分析,研究该基因对棉纤维中结构多糖和苯丙烷类化合物含量及纤维中苯丙烷类结构单体的影响。结果显示:(1)GhCAD6基因以单拷贝的形式整合到受体棉花基因组中。(2)转基因植株纤维中GhCAD6基因的表达量低于相同发育阶段对照样品,在对照样品中GhCAD6基因的表达量表现为先升高,在20 DPA(开花后天数)时表达量最高,之后下降,而在转基因植株纤维中先上升,并于发育15 DPA时表达量下降,20 DPA时又上升至最高,之后再次下降。(3)成熟纤维中,转GhCAD6基因植株纤维中苯丙烷类化合物含量低于对照,但结构多糖含量的差别不明显。(4)转GhCAD6基因纤维中苯丙烷类结构单体——紫丁香基木质素(S 木质素)和愈疮木基木质素(G 木质素)的比值下降。研究表明,棉花转入GhCAD6基因后,纤维发育(15 DPA)中GhCAD6基因的表达量变化可能导致棉花中苯丙烷类化合物含量及其结构单体比率变化,从而造成棉花纤维品质改变。该研究结果可为深入分析GhCAD6基因在改良棉花纤维品质的作用机理提供理论依据。
中文关键词:棉花纤维  苯丙烷代谢  GhCAD6基因  纤维品质  结构多糖  苯丙烷类化合物
 
Expression of GhCAD6 Gene in Cotton Fiber and the Effect on Structural Components
Abstract:The material is ‘Xinluzao 36’ transformed with GhCAD6 gene obtained by Agrobacterium mediated transformation. In this study, the T6 generation of the transgenic material was used as experimental material. The integration of GhCAD6 gene in the genome of cotton, the expression in different developmental stages of cotton fiber, the content of structural polysaccharides and phenylpropanoid compounds in the cotton fiber, and the influence of the fiber in the phenylpropanoid monomer were analyzed. The results showed: (1) GhCAD6 was integrated in the form of single copy to the recipient cotton genome. (2) The expression level of GhCAD6 in the transgenic plants was lower than that in the control samples at the same developmental stage. The expression of GhCAD6 was increased firstly, reached the highest at 20 DPA and then decreased in the control samples. While the expression decreased at 15 DPA in the transgenic plant fibers, rose to the highest level at 20 DPA, and then decreased. (3) In mature fibers, the content of phenylpropanoid compounds in the transgenic plants was lower than that in the control samples, and the content of structural polysaccharides had no significant difference. (4) The ratio of S/G decreased in cotton fiber of transgenic plants. After the GhCAD6 gene transferred into cotton, the change of GhCAD6 gene expression at 15 DPA may be the cause of the change of content of phenylpropanoid compounds and ratio of structural monomer, which result in the variation of cotton fiber quality. The results of this research can provide theoretical basis for further analysis of the mechanism of GhCAD6 gene in improving cotton fiber quality.
keywords:cotton fiber  phenylpropanoid metabolism  GhCAD6 gene  fiber quality  structural polysaccharides  phenylpropanoid compounds
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