Abstract:We investigated pollinia development in rare orchid Vanda coerulea using paraffin sectioning in order to provide some embryological data to clarify the generic delimitation of AeridesVanda alliance. Results are as follows: (1) initially anther primordia were differentiated into a pair of thecae containing two microsporangia. And then, a sterile septum was formed longitudinally and unequally in each microsporangium during microsporogenesis. Finally, the septum was totally absorbed leading to 2 cleftpollinia at ripen anther stage. (2) The development of anther wall was of massive type due to 5-9 layers including a transient epidermis, 2-6 endothecium layers, a middle layer and a uninucleated tapetum. (3) Microspore mother cells underwent a successive cytokeinsis during meiosis resulting in some tetrahedral or isobilateral microspore tetrads. Microspores retained in tetrads and developed into 2celled pollen tetrads. The pollen tetrads arranged compactly within the collapsed anther wall and formed 2 cleftpollinia in anther. These embryological features provided new data to the taxonomy of Vanda S. S.