2种杓兰属植物内生狭截盘多毛孢菌的分离与鉴定
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引用本文:徐玲玲,赵桂仿,孙敬祖,张 焱,陶贵荣.2种杓兰属植物内生狭截盘多毛孢菌的分离与鉴定[J].西北植物学报,2019,39(7):1316~1321
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作者单位
徐玲玲1,赵桂仿2*,孙敬祖3,张 焱1,陶贵荣1 (1 西安文理学院 生物与环境工程学院植物微生物协同研究重点实验室西安 7100652 西北大学 生命科学学院西部资源生物与现代生物技术教育部重点实验室西安 7100693 中国科学院 微生物研究所真菌学国家重点实验室北京 100101) 
基金项目:国家自然科学基金(31300366);
中文摘要:该文报道了西藏杓兰(Cypripedium tibeticum)和无苞杓兰(C. bardolphianum)根中内生真菌新记录种——狭截盘多毛孢(Truncatella angustata)。该研究从四川黄龙沟不同海拔区分别采集到西藏杓兰和无苞杓兰,并在其根中分离获得148个狭截盘多毛孢菌株。形态学观察显示,狭截盘多毛孢菌株在PDA培养基上菌落白色,背面黄褐色;分生孢子器黑色脓包状;分生孢子梭型,4个细胞,3个隔膜;顶端附属丝1~5根。ITS rDNA分析发现3种新基因型菌株(HLIO15_15a_22、HLIO15_20a_42和HLIO15_17a_46);在海拔最高(3 330~3 400 m)的居群中狭截盘多毛孢的分离率最高,且包含全部3种基因型菌株。构建截盘多毛孢属(Truncatella)真菌ITS序列系统发育树,发现狭截盘多毛孢不同生态功能的菌株没有形成明显分支。
中文关键词:狭截盘多毛孢  兰科植物  形态  生态功能
 
Isolation and Identification of Endophytic Truncatella angustata in Roots of Two Cypripedium Species
Abstract:One new record of endophytic fungus, Truncatella angustata, in roots of Cypripedium tibeticum and C. bardolphianum, was reported in this paper. 148 strains of Truncatella angustata were isolated from the roots of C. tibeticum and C. bardolphianum as endopytes at different altitudinal areas in Huanglong Valley, Sichuan Province, China. Morphological descriptions of T. angustata revealed that the colony on PDA pale yellow, reverse of the culture yellowish brown; pycnidia black, pustule like; conidia fusiform, 4 celled, 3 septate; apical appendages 1-5. Three genotypes (HLIO15_15a_22, HLIO15_20a_42 and HLIO15_17a_46) were identified by ITS rDNA analysis. The isolation rate of T. angustata was highest in the highest altitude population, containing all three genotype strains. The phylogenetic tree of the ITS sequence of the genus Truncatella was constructed. It was found that the strains with different ecological functions of the genus Truncatella did not form obvious clades.
keywords:Truncatella angustata  Orchidaceae  morphology  ecological function
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