白菜PLD基因家族全基因组鉴定及对高温胁迫的响应
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引用本文:张艺思,刘高峰,刘路平,侯喜林.白菜PLD基因家族全基因组鉴定及对高温胁迫的响应[J].西北植物学报,2019,39(8):1361~1370
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张艺思,刘高峰,刘路平,侯喜林* (南京农业大学 作物遗传与种质创新国家重点实验室农业部华东地区园艺作物生物学与种质创制重点实验室南京 210095) 
基金项目:国家现代农业产业技术体系项目(CARS 23 A 06)
中文摘要:膜磷脂是产生胞内信号信使的重要来源,磷脂酶Ds(phospholipase D,PLDs)可以催化磷脂产生信号分子磷脂酸(phosphatidic acid, PA),从而响应不同胁迫信号。该研究利用生物信息学方法对白菜PLD基因家族的基因成员进行鉴定及结构域分析,并采用qRT PCR方法对不结球白菜的18个BrPLD基因的表达进行分析,以探讨不结球白菜的BrPLD基因家族对高温胁迫的响应机制。结果表明:(1)共鉴定到18个白菜PLD基因家族成员,其中有2个成员(BrPLD03和BrPLD09)的C2结构域被替换为PH/PX结构域,另有1个基因(BrPLD12)缺少了其N末端保守结构域,由信号肽代替。(2)根据编码蛋白结构域的不同,18个BrPLD基因分为3个亚类,分别为15个C2 BrPLD、2个PH/PX BrPLD和1个SP BrPLD;氨基酸理化性质分析发现,该基因家族编码蛋白多半为酸性蛋白;18个基因分布在除4号和7号之外的8条染色体,且呈现不均匀分布,并发现了BrPLDs蛋白Ca2+配位碱基的缺失。(3)qRT PCR检测发现,高温处理下不结球白菜的BrPLD基因的表达量发生明显变化,各基因在耐热和热敏品种中的表达具有差异。(4)对BrPLD基因家族不同功能顺式响应元件的预测发现,所有家族基因含有光应答有关的作用元件,9个基因含有与低温有关的顺式元件,10个基因含有调控干旱相关元件,18个基因均未预测到热胁迫相关顺式响应元件。
中文关键词:白菜  基因家族  生物信息  高温胁迫
 
Genome wide Identification of PLD Gene Family in Chinese Cabbage and Its Response to High Temperature Stress
Abstract:Membrane phospholipids are important sources of intracellular signal messengers and phosphatidic acid (PA) can be catalyzed by phospholipase Ds (PLDs) in response to different stress signals. In this study, we used Bioinformatics to identify and analyze the gene members of PLD gene family in Chinese cabbage; and the qRT PCR to analyze the expression of 18 BrPLD genes in non heading Chinese cabbage, in order to explore the response mechanism of BrPLD gene family in non heading Chinese cabbage to high temperature stress. The results showed that: (1) a total of 18 PLD gene family members were identified. Two of them (BrPLD03 and BrPLD09) were replaced by PH/PX domain. Another gene(BrPLD12)lacked its N terminal conservative domain and was replaced by signal peptide. (2) Depending on the domain of the encoding protein,18 BrPLD genes were classified into three subgroups: 15 C2 BrPLD, 2 PH/PX BrPLD and 1 SP BrPLD;analysis of physicochemical properties of amino acids revealed that most of the proteins encoded by the gene family were acidic proteins; 18 genes were distributed on another 8 chromosomes except chromosomes 4 and 7, and showed non uniform distribution. The deletion of Ca2+ ligand base of BrPLDs protein was also found.(3) Quantitative RT PCR analysis of non heading Chinese cabbage under high temperature showed that BrPLD gene could respond to high temperature stress, and the expression of BrPLD gene changed significantly. The expression of BrPLD gene was different in heat tolerant and heat sensitive varieties. (4) Prediction of cis response elements with different functions in BrPLD gene family revealed that all family genes contained light related action elements, 9 genes contained cis response elements related to low temperature, 10 genes contained drought related elements, all 18 genes did not predict heat stress related cis response elements. The results of this study have important reference value for further studying the mechanism of BrPLD gene family responding to high temperature stress.
keywords:Chinese cabbage  gene family  bioinformatics  high temperature stress
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