马铃薯糖转运蛋白基因的克隆及表达分析
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引用本文:张 璐,李 明,叶广继,贺苗苗,王 舰,纳添仓.马铃薯糖转运蛋白基因的克隆及表达分析[J].西北植物学报,2019,39(9):1528~1533
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张 璐1,李 明1,叶广继1,2,3,4,5,贺苗苗1,2,3,4,5,王 舰1,2,3,4,5,纳添仓1,2,3,4,5* (1 青海大学西宁 8100162 青海省农林科学院西宁 8100163 青海大学省部共建三江源生态与高原农牧业国家重点实验室西宁 8100164 青藏高原生物技术教育部重点实验室西宁 8100165 青海省马铃薯育种重点实验室西宁 810016) 
基金项目:中国科学院“西部之光”人才培养引进计划;
中文摘要:植物SWEET基因家族是一类糖转运蛋白,在植物的生理活动和生长发育过程中发挥着重要功能。为了解马铃薯SWEET基因的相关信息,探究其在马铃薯不同组织以及在生物胁迫与非生物胁迫下的表达特性。该研究采用同源克隆技术从马铃薯‘青薯9号’中克隆了StSWEET5基因(GenBank登录号为MN295671),其CDS序列长度为717 bp,编码238个氨基酸。系统进化树分析结果表明,StSWEET5与番茄的氨基酸序列相似性最高(97.06%)。qRT PCR分析表明:StSWEET5基因在马铃薯各组织(根、茎、叶、花、块茎、匍匐茎)中均有表达,且在花中的表达显著高于其他组织;糖胁迫下,StSWEET5基因在根、茎、叶中均有表达,尤其在根中的表达差异最为显著(P<0.05)。在晚疫病菌(Phytophthora infestans)诱导后36 h时,表达量达到最高,随后急剧下调。推测StSWEET5基因参与了马铃薯糖胁迫以及响应了晚疫病诱导的过程。
中文关键词:马铃薯  StSWEET5  克隆  糖胁迫  晚疫病诱导
 
Cloning and Expression Analysis of a Sugar Transporter Protein Gene in Potato
Abstract:Plants SWEET gene family is a kind of sugar transporter, which plays an important role in plant physiological activities and growth and development.In order to understand the information related to potato SWEET gene and explore its expression characteristics in different potato tissues and under biological and abiotic stress, we cloned the StSWEET5 gene from potato ‘Qingshu 9’ using homologous cloning technology(GenBank accession number is MN295671), whose CDS sequence length was 717 bp and encoded 238 amino acids.Phylogenetic tree analysis showed that the amino acid sequence similarity between the protein and that of tomato was the highest, reaching 97.06%. The results of qRT PCR showed that StSWEET5 gene was expressed in all tissues of potato (root, stem, leaf, flower, tuber and stolon), with the highest expression in flowers. StSWEET5 can be expressed in rhizomes and leaves under sugar stress, especially in roots (P<0.05). After 36 h induction of Phytophthora, the expression of Phytophthora in leaves was significantly uprated. It is speculated that StSWEET5 gene is involved in potato sugar stress and responds to late blight induction.
keywords:potato  StSWEET5  clone  carbohydrate stress  Phytophthora infestans induction
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