紫背天葵叶和花中花青素合成相关转录组基因分析
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引用本文:张少平,郑开斌,洪佳敏,林宝妹,张 帅,邱珊莲.紫背天葵叶和花中花青素合成相关转录组基因分析[J].西北植物学报,2019,39(9):1551~1558
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张少平1,2,郑开斌1,2,洪佳敏1,林宝妹1,张 帅1,邱珊莲1* (1 福建省农业科学院 亚热带农业研究所福建漳州 3630052 福建省农业科学院 作物研究所福州350013) 
基金项目:福建省农业科学院创新团队(STIT2017 2 11);
中文摘要:为获取紫背天葵(Gynura bicolor D C.)花青素合成代谢相关调控基因信息,该试验以紫背天葵叶片为材料,以其花朵为对照,进行转录组测序,并进行CHS、CHI、F3H等8类合成酶基因以及MYB、bHLHWD40等3类转录因子检索,从中选取8个相关差异表达显著调控基因进行qRT PCR验证分析。结果显示:(1)在紫背天葵中共获得72个花青素合成酶信息,其中差异表达明显的有1个F3′H和2个3GT下调,9个F3H基因中有上调基因4个和下调基因5个。(2)在紫背天葵中获取到238个MYB、113个bHLH和219个WD40转录因子,这3类转录因子中差异表达明显的分别为22个、16个和7个。(3)qRT PCR结果显示,所选取的8个花青素合成相关调控基因,在紫背天葵叶及花朵中的下调表达趋势与转录组测序结果完全一致,但不同基因差异表达趋势略有不同。研究表明,在紫背天葵叶片和花朵中所存在的大量花青素合成代谢调控基因中,只有少量差异表达显著,但转录因子相比合成酶的调控更为复杂。
中文关键词:紫背天葵  转录组基因  花青素  调控基因  合成酶  转录因子
 
Transcriptome Analysis of Anthocyanin Synthesis Genes in Gynura bicolor Leaves and Flower
Abstract:In order to explore anthocyanin synthsis regulator genes in Gynura bicolor, we sequenced the transcriptomes of G. bicolor including leaf and flower in control by Illumina HiSeq 2 500 platform. Then, 8 kinds of synthases such as CHS, CHI, F3H, F3′H, F3′5′H, DFR, ANS, 3GT and 3 kinds of transcription factors such as MYB, bHLH and WD40 were retrieved. At the same time, 8 differential expressed genes were identified by the RT PCR. The results showed that: (1) There were 72 unigenes related to anthocyanin synthases, in which 12 differential expressed genes including 1 down regulated F3′H, 2 down regulated 3GTs, 5 down regulated F3Hs and 4 up regulated F3Hs. (2) There were 238 MYBs, 113 bHLHs and 219 WD40s. The differential expressed genes in these 3 kinds of transcription factors were 22, 16 and 7, respectively. (3) According to transcriptome sequencing, the down expression trend of these 8 anthocyanin synthesis regulator genes agreed with the testing by the RT PCR. But the trend multiples of these differentially expressed genes by these 2 kinds of approaches were different. So this study concluded that there are many anthocyanin synthesis regulator genes in G. bicolor including leaf and flower, but only a few of these genes are significantly different. At the same time, the regulation of transcription factors is more complex than that of synthases.
keywords:Gynura bicolor  transcriptome gene  anthocyanin  regulator gene  synthase  transcription factor
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