中国古老月季‘月月粉’FT/TFL1基因的鉴定与表达分析
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引用本文:吴高琼,李淑斌,王炜佳,张 颢,唐开学,王其刚.中国古老月季‘月月粉’FT/TFL1基因的鉴定与表达分析[J].西北植物学报,2019,39(10):1741~1749
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作者单位
吴高琼1,2,李淑斌1,王炜佳1,2,张 颢1,唐开学1,2,王其刚1* (1 云南省农业科学院 花卉研究所国家观赏园艺工程技术研究中心, 昆明 650205
2 云南大学 生命科学学院, 昆明 650091) 
基金项目:国家自然科学基金项目(31660583);
中文摘要:该研究以中国古老月季品种‘月月粉’(Rosa chinensis ‘Old Blush’)为试材,根据拟南芥和草莓FT/TFL1基因家族成员的保守结构域设计引物,对‘月月粉’基因组DNA进行克隆和测序,结合数据库序列和双单倍体基因组信息进行序列比对,结果共获得了8条月季的FT/TFL1基因家族序列,且8条序列分别注释为2个FT基因(RcFT1和RcFT2)、1个BFT基因(RcBFT)、1个ATC基因(RcATC)、2个MFT基因(RcMFT1和RcMFT2)和2个PEBP基因(PEBP1和PEBP2);进化树分析结果将这8个基因分为3个亚组;结构分析显示8个基因的内含子为1 ~ 5个不等。qRT PCR分析显示,RcFT1、RcPEBPRcMFT1、RcMFT2和RcBFT在‘月月粉’的叶片中表达量最高,茎尖中次之;RcFT2在茎尖中的表达最高,叶片中次之;RcATC在茎尖中的表达量高于其他组织;除RcPEBP在根组织中有表达外,其他基因在根组织中几乎没有检测到。进一步表达分析显示,RcFT1和RcFT2在‘无刺光叶蔷薇’(R. wichuriana ‘Basyes Thornless’)生殖生长期的叶片、茎尖中的表达量显著高于营养生长期。研究推测,具有连续开花性状的中国古老月季‘月月粉’可能具有多个开花时间(FT)基因位点,FT有可能可以作为月季生殖转换的标记基因。
中文关键词:中国古老月季  FT/TFL1基因  开花时间  基因表达
 
Identification and Expression Analysis of the FT/TFL1 Genes in Rosa chinensis ‘Old Blush’
Abstract:We used the Chinses old garden rose Rosa chinensis ‘Old Blush’ as test material and designed the degenerate primers according to the conserved domains of the FT/TFL1 gene family members of Arabidopsis thaliana and Fragaria vesca. We cloned and sequenced the genomic DNA of R. chinensis ‘Old Blush’, and did the sequence alignment based on database sequence and double haploid genomic information. We obtained a total of 8 FT/TFL1 gene family sequences, and the 8 sequences were respectively annotated as 2 FT genes (RcFT1 and RcFT2), 1 BFT gene (RcBFT), 1 ATC gene (RcATC), 2 MFT genes (RcMFT1 and RcMFT2), and 2 PEBP genes (PEBP1 and PEBP2). Phylogenetic tree analysis divided these 8 genes into 3 sub groups. Structural analysis showed that the introns of 8 genes ranged from 1-5. qRT PCR expression analysis FT/TFL1 genes in different tissues of R. chinensis ‘Old Blush’ showed that RcFT1, RcPEBP, RcMFT1, RcMFT2 and RcBFT have the highest expression in leaves, followed in stem tips. RcFT2 had the highest expression level in stem tips and second in leaves. The expression of RcATC in stem tips was the highest, while lower in other tissues. RcPEBP gene has expression in root tissues, other genes have no expression in roots. In addition, the expression level of RcFT1 and RcFT2 in the leaves and stem tips of R. wichuriana ‘Basyes Thornless’ during the reproductive growth period were significantly higher than those during the vegetative growth period. We speculated that the R. chinensis ‘Old Blush’ with continuous flowering may have multiple flowering time (FT) gene loci, and FT may be used as a marker gene for reproductive transformation of rose.
keywords:Chinses old garden roses  FT/TFL1 genes  flowering time  gene expression
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