In this experiment, the‘Hongyan’strawberry fruit (Fragaria ananassa Duch.) was used as test material, and salicylhydroxamic acid (SHAM) was used to inhibit the mitochondrial alternative pathway to make the cytochrome pathway run alone. The mitochondrial extract was treated with electron transfer and oxidative phosphorylation coupling accelerators ［adenosine diphosphate (ADP) and succinic acid disodium salt (C4H₄Na₂O₄)］ and inhibitors ［2,4dinitrophenol (DNP) and sodium vanadate solution (NaVO₄)］. Under the condition of promoting and inhibiting respiratory metabolism, the changes of mitochondrial respiratory metabolism and ultra weak luminescence (UWL) and the relationship between them were compared and analyzed in strawberry fruit.The results showed that: (1) the mitochondria of strawberry fruit were treated with accelerator, the key enzymes activity of respiratory metabolism including succinate dehydrogenase (SDH), cytochrome oxidase (COX), ATP synthase (H⁺ATPase), respiration rate and adenosine triphosphate (ATP) content increased with the increase of concentration, and the intensity of UWL increased with the increase of concentration. The respiratory metabolic index and UWL intensity at each concentration were higher than those of the control. After treatment with inhibitor, the changes of respiratory metabolism indexes and UWL intensity were opposite to those of accelerator, and all of them were lower than those of the control. (2) The correlation analysis showed that all indexes of respiratory metabolism were positively correlated with UWL.The above results showed that the mitochondrial UWL intensity of strawberry fruit changed with the change of respiratory metabolism; the accelerator promoted the respiratory metabolism and led to the increase of UWL intensity; on the contrary, the inhibitor inhibited respiratory metabolism and led to the decrease of UWL intensity; It suggested that mitochondria was one of the organelles that produced UWL, and UWL was stimulated in the process of mitochondrial respiration and metabolism.