苦荞R2R3 MYB转录因子调控原花青素生物合成的研究
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引用本文:王 霜,雒晓鹏,姚英俊,杨静静,陈 英,伍 琼,吴 琦.苦荞R2R3 MYB转录因子调控原花青素生物合成的研究[J].西北植物学报,2019,39(11):1911~1918
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作者单位
王 霜,雒晓鹏,姚英俊,杨静静,陈 英,伍 琼,吴 琦* (四川农业大学 生命科学学院四川雅安 625014) 
基金项目:四川省科技厅国际合作计划(2015HH0047)
中文摘要:基于苦荞花期转录组数据,该研究筛选并克隆获得一个黄酮代谢相关的MYB类转录因子,并命名为FtMYB23。该基因ORF框长879 bp,编码292个氨基酸;系统进化树分析显示,FtMYB23与SG5 MYB亚家族成员聚为一簇,属于典型的R2R3 MYB型转录因子。β 半乳糖苷酶滤纸分析表明,其具有转录激活活性。FtMYB23过表达转基因拟南芥株系的表型分析表明,3个阳性株系的种皮颜色均呈现出比野生型更深的褐色,其叶中原花青素含量均极显著增加(P < 0.01),分别为野生型的4.68、3.5和2.8倍。qRT PCR分析表明,转基因拟南芥中黄酮合成相关的AtCHSAtCHIAtF3HAtF3′HAtFLSAtDFRAtBAN等基因的表达量显著升高(P < 0.05),而AtTT12的表达量极显著降低(P < 0.01)。研究认为,FtMYB23作为典型的Subgroup5 MYB(SG5 MYB)激活型转录因子,通过促进黄酮合成途径早期关键酶基因的表达,从而提高原花青素的合成与积累。
中文关键词:苦荞  R2R3 MYB  转录因子  原花青素
 
Characterization of an R2R3 MYB Transcription Factor Involved in the Synthesis of Proanthocyanidins from Tartary Buckwheat
Abstract:In this study, a MYB transcription factor related to flavonoids metabolism was screened from florescence transcriptome data of Tartary buckwheat, which was named FtMYB23. Bioinformatic analysis showed that it contains a 879 bp ORF that encodes a protein of 292 amino acids. Multiple sequence analysis presented that FtMYB23 is a typical R2R3 MYB transcription factor. Phylogenetic tree indicated that FtMYB23 should be gathered into the same cluster with SG5 MYB subfamily members. Analysis of β galactosidase filter paper showed transcriptional activation activity. Transgenic lines exerted increased pigmentation in seed coats, and the content of proanthocyanidins in leaves was significantly increased 4.68, 3.5 and 2.8 times of wild type, respectively (P < 0.01). Meanwhile, the expression of AtCHS, AtCHI, AtF3H, AtF3′H, AtFLS, AtDFR, AtBAN genes related to flavonoids synthesis in transgenic Arabidopsis thaliana was significantly upregulated (P < 0.05). Nevertheless, AtTT12 was dramatically down regulated (P < 0.01). Therefore, as a typical Subgroup5 MYB (SG5 MYB) activating transcription factor, FtMYB23 promotes the expression of key enzymes in the early stage of flavonoid biosynthesis pathway, thereby enhancing the synthesis and accumulation of proanthocyanidins.
keywords:tartary buckwheat(Fagopyrum tataricum)  R2R3 MYB  transcription factor  proanthocyanidin
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