马铃薯HSP17.7基因的克隆及其对高温逆境的响应
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引用本文:奚玉培,张志忠,王景荣,韩晓云,Gefu WANG PRUSKI.马铃薯HSP17.7基因的克隆及其对高温逆境的响应[J].西北植物学报,2019,39(11):1952~1960
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奚玉培1,张志忠1,王景荣1,韩晓云1,Gefu WANG PRUSKI1,2* (1 福建农林大学 园艺学院福建农林大学 戴尔豪西大学联合实验室福州 3500022 戴尔豪西大学 农学院加拿大特鲁罗 B2N 5E3) 
基金项目:福建省闽江学者科研基金(116 114120019);
中文摘要:热激蛋白和植物对高温的响应密切相关,同时在植物生长发育调控和逆境抵抗等方面具有重要作用。该研究克隆了马铃薯热激蛋白基因StHSP17.7(GenBank登录号为XP_006350804.1),对其全长cDNA序列进行了相关生物信息学分析,并利用qRT PCR分析StHSP17.7基因在高温胁迫下的差异表达特性。结果显示:(1)StHSP17.7全长755 bp,包含465 bp开放阅读框,编码154个氨基酸。(2)StHSP17.7分子质量约为17.62 kD,等电点7.91,为亲水性蛋白,C端含有保守序列Ⅰ和Ⅱ组成的ACD结构域,属于典型的sHSPs家族成员。(3)系统进化分析发现马铃薯小分子热激蛋白归为12个亚家族,其中StHSP17.7与马铃薯HSP17.6蛋白亲缘关系较近,属于小分子热激蛋白C的Ⅰ类家族成员;基因结构分析显示,在马铃薯48个sHSP基因中,StHSP17.7基因不含内含子,含1个内含子的基因有23个,占47.9%。(4)qRT PCR分析显示,高温能够快速诱导StHSP17.7基因表达,且基因表达量呈爆发式变化,在24 h达到最高值。研究表明,StHSP17.7基因明显参与了马铃薯对高温的响应。
中文关键词:马铃薯  StHSP17.7基因  高温逆境  小分子热激蛋白
 
Cloning of HSP17.7 Gene and Its Response to High Temperature Stress in Potato
Abstract:Plant heat shock proteins are representative proteins that respond to high temperatures and play an important role in regulating plant growth and development and resisting adversity. In this study, HSP17.7 gene from Solanum tuberosum was cloned by RT PCR and bioinformatic characters were analyzed. The expression level of StHSP17.7 gene under high temperature stress was analyzed by qRT PCR. The results showed that: (1) full length sequence of StHSP17.7 gene was 755 bp. It contains an open reading frame of 465 bp and 154 amino acids were encoded. (2) The StHSP17.7 gene encoding protein was a hydrophilic protein with molecular weight of 17.62 kD and the isoelectric point is 7.91. The functional structure domain analysis showed that the C terminal of the protein contained an ACD domain consisting of conserved sequence Ⅰ and conserved sequence Ⅱ, typically belonging to the sHSPs family member. (3) Phylogenetic analysis showed that sHSPs of S. tuberosum was clustered into 12 subfamilies. The StHSP17.7 protein was closely related to the HSP17.6 protein of S. tuberosum, and belongs to the family of small heat shock protein C Ⅰ. Gene structure analysis showed that among 48 StHSPs genes from S. tuberosum, StHSP17.7 gene does not contain intron, and other 23 genes contained one introns, accounting for 47.9% of all the genes in the family. (4) qRT PCR analysis showed that StHSP17.7 gene expression was rapidly induced by high temperatures, and the gene expression level showed an explosive change, reaching the highest value in 24 h. The results suggest that StHSP17.7 gene is very sensitive to heat stress and may play an important role in response to high temperature stress in potatoes.
keywords:Solanum tuberosum L.  StHSP17.7 gene  high temperature stress  small heat shock proteins
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