By employing the method of using space as a substitute for time and continuum index, we analyzed the plant species composition of sixty representative typical plots and the height, density, biomass, Shannon-winner index, Pielou index and Simpson index of plant communities at the different succession stages in the Dolomite karst area of southeastern Guizhou Province, in order to explore the evolution characteristics of plant communities and get into the foundation for the natural restoration rules of plant communities in the karst area of Dolomite. The results showed that: (1) the dominant species of each group are composed differently, the substitution rules of the group are pioneer species, sub-pioneer species, transition species, and finally replaced by the climax species. (2) The structural characteristics of height, density and biomass of the communities gradually increased with the succession of plant communities, with the range of change being 0.58-9.54 m and 585-3 145 plants·hm^-2 and 8.45-128.56 t·hm^-2, respectively. (3) The species richness of plant communities decreased with the development of succession stages, and the number of species from herbaceous stage to climax stage decreased from 48 species to 10 species. (4) With the development of plant communities succession stages, the shannonwinner index increased firstly and then decreased, from 3.48 in herbaceousscrub stage to 4.73, and then decreased to 3.46 in climax stage; The Pielou index showed a decreasing trend, with the highest being 0.95 in herbaceousscrub stage and the lowest being 0.78 in the evergreen and deciduous stage; The Simpson index gradually increased and reached the maximum 0.10 in the climax stage.