Abstract:CBLinteracting protein kinase (CIPK) is a family of specific serine/threonine (Ser/Thr) protein kinases in plants, which participates in response to various biotic and abiotic stresses. In this study, the fulllength cDNA of Jatropha curcas JcCIPK2 gene was cloned by RTPCR, and the expression patterns of JcCIPK2 in different tissues and treatments (low temperature at 12 ℃ and 1 ℃, high temperature at 42 ℃, 30% PEG, 250 mmol/L NaCl, 150 μmol/L ABA) were analyzed by fluorescence quantitative qRTPCR. The results showed that: (1) The open reading frame of JcCIPK2 gene was 1 398 bp, encoding 465 amino acids, the relative molecular weight was 52.95 kD, and the isoelectric point was 8.89. (2) Protein structure analysis indicated that the Nterminus of JcCIPK2 contains serine/threonine kinase_sucrose nonfermenting1 related protein kinase 3 catalytic domain STKc_SnRK3, which located between the 11th and 265th amino acids, and with an activation loop in the kinase domain; Cterminal contains the CIPK protein kinaseregulated domain CIPK_C, between the 316th and 430th amino acids, and its regulatory domain also contains a NAF domain which is specifically associated with CBL, and is typical of the CIPK family, between the 314th and 369th amino acids. (3) Phylogenetic analysis showed that the JcCIPK2 protein has the highest homology with Manihot esculenta belonging to the Euphorbiaceae, and the sequence identity is up to 87%. (4) JcCIPK2 gene was expressed in roots, stems and leaves of J. curcas. After treatment at 12 ℃ and 1 ℃ the expression of JcCIPK2 gene in leaves was upregulated and then downregulated. The highest expression level was observed at 24 h under the low temperature treatment, and was increased by 6.0 and 16.72 folds, respectively, compared with the control. In addition, JcCIPK2 gene was induced to different levels by treatments with NaCl (250 mmol/L), high temperature (42 ℃), ABA (150 μmol/L), and drought stress (30% PEG). These results indicated that JcCIPK2 gene plays important roles in the response and adaptation of J. curcas to various stresses.