檀香SaRbohA基因的克隆与吸器诱导因子响应分析
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引用本文:孟 森,马海宾,王胜坤,李振双,赵 忠,陆俊锟.檀香SaRbohA基因的克隆与吸器诱导因子响应分析[J].西北植物学报,2019,39(12):2132~2137
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作者单位
孟 森1,马海宾1,王胜坤1,李振双1,赵 忠2,陆俊锟1* (1 中国林业科学研究院 热带林业研究所/国家林业和草原局热带林业研究重点实验室广州 5105202 西北农林科技大学 林学院陕西杨陵 712100) 
基金项目:中国林科院基本科研业务费(CAFYBB2017SY020);
中文摘要:植物Rboh基因家族编码产生活性氧(ROS)的NADPH氧化酶。了解半寄生植物檀香(Santalum album Linn.)基因Rboh相关信息和表达特性,可为研究檀香SaRbohA基因通过活性氧信号(ROS)调控吸器发育的响应提供理论依据。该研究以全长转录组测序为基础,通过序列拼接设计合成基因特异引物,从根中克隆获得了1个檀香respiratory burst oxidase homolog(Rboh)基因cDNA全长,命名为SaRbohA。序列分析表明,该基因cDNA全长2 790 bp,编码929个氨基酸,分子量105.37 kD,理论等电点9.13,预测亚细胞定位于细胞膜。结构预测表明,SaRbohA具有6个跨膜结构域,在膜内侧的C端包含典型的NADPH结合结构域和FAD结合结构域, N端含有两个EF手性结构。序列比对分析表明,檀香SaRbohA与苹果MdRboh同源进化关系较近,相似度为63.65%。组织特异性表达分析表明,SaRbohA基因在茎中表达量最低,幼叶和茎尖中表达量较高,而在根中表达量最高。采用2, 6 二甲氧基对苯醌(寄生植物吸器诱导因子)处理,可以强烈诱导檀香SaRbohA基因的响应并伴随大量活性氧信号。研究推测,SaRbohA基因的在ROS信号介导的檀香吸器发育过程中起重要作用,且受化学诱导因子调控表达。
中文关键词:寄生植物  檀香  SaRbohA  基因克隆  活性氧(ROS)
 
Cloning and the Haustorium Inducing Factor Response Analysis of SaRbohA Gene in Santalum album Linn.
Abstract:Plant Rboh gene family encodes NADPH oxidases. The information and expression characteristics related to SaRboh genes in sandalwood (Santalum album Linn.) will contribute to study the roles of Rboh in sandalwood growth and development. Based on the full length transcriptome data, the cDNA sequence of SaRbohA (respiratory burst oxidase homolog) gene was obtained from roots of sandalwood. The full length of SaRbohA is 2 790 bp, encoding 929 amino acids with molecular weight 105.37 kD and isoelectric point 9.13. Subcellular localization analysis showed that SaRbohA localized on the cell membrane. Sequence alignment indicated SaRbohA has six transmembrane domains; The protein includes a typical NADPH binding domain and a FAD binding domain in C terminal, and two EF hand structures in N terminal. Phylogenetic tree analysis showed that the amino acid sequence similarity between SaRbohA and MdRboh of apple was the highest, reaching 63.65%. Tissue specific expression analysis showed that the SaRbohA gene was low expression in stems, high expression in young leaves and apical buds, especially in roots of sandalwood. 2, 6 dimethoxy ρ benzoquinoe (an important haustorium inducing factor) can strongly induce the expression of SaRbohA. SaRbohA may play important roles in the development of ROS mediated haustorium development and be induced by the chemical factor.
keywords:parasitie  Santalum album Linn.  SaRbohA  gene clone  reactive oxygen species (ROS)
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