牡丹开花相关SWEET家族基因生物信息学与表达模式分析
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引用本文:王 锐,徐娟娟,刘 鑫,逯久幸,栗 燕.牡丹开花相关SWEET家族基因生物信息学与表达模式分析[J].西北植物学报,2019,39(12):2145~2153
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作者单位
王 锐,徐娟娟,刘 鑫,逯久幸,栗 燕* (河南农业大学 林学院,郑州 450002) 
基金项目:河南省科技攻关(192102110062);
中文摘要:为了揭示SWEET家族基因在牡丹开花过程中的作用,该研究以‘洛阳红’牡丹花瓣转录组数据库为基础,利用生物信息学方法对SWEET家族基因进行鉴定和分析。结果表明:(1)实验共得到10个具有完整开放阅读框的牡丹SWEET基因。(2)牡丹SWEET家族成员蛋白等电点、消光系数等差别不大,其中5个牡丹SWEET基因编码的蛋白为不稳定蛋白;亚细胞定位预测这10个牡丹SWEET基因编码的蛋白均定位在细胞膜;进化树分析显示,牡丹SWEET基因与拟南芥亲缘关系更近;结构分析表明,牡丹SWEET蛋白结构在进化过程中非常保守,这10个牡丹SWEET蛋白同时具有5个相同的Motif且均含2个MtN3 saliva结构域。(3)可溶性糖含量分析显示,从小风铃期到盛花期,牡丹花瓣中蔗糖含量不断降低,果糖和葡萄糖含量不断升高,均在盛花期达到峰值。(4)qRT PCR分析显示,PsSWEET4盛花期表达量是小风铃期的34倍, PsSWEET8盛花期表达量是小风铃期的60倍。结合这2个基因表达与进化关系及可溶性糖变化值,初步推测PsSWEET4和PsSWEET8在开花过程中可能通过调控果糖、葡萄糖的转运而间接调控开花过程;该结果为进一步研究PsSWEET基因在牡丹生长发育过程中的功能奠定了基础。
中文关键词:牡丹  SWEET基因  生物信息学  表达分析
 
Bioinformatics and Expression Pattern Analysis of SWEET Family Genes during Peony Flowering Development
Abstract:We investigated the function of SWEET family genes in the flowering process of tree peony. Based on the ‘Luoyang red’ tree peony petal transcriptome database, the SWEET family genes were identified and analyzed by bioinformatics. The results showed that: (1) a total of 10 SWEET genes with complete open reading frame were obtained. (2) There were little difference in isoelectric point and extinction coefficient of peony SWEET family proteins, among which 5 peony SWEET genes encoded proteins were unstable proteins. Subcellular localization predicted that the 10 SWEET genes of tree peony were located in the cell membrane. Evolutionary tree analysis showed that the SWEET genes of tree peony was more closely related to Arabidopsis thaliana. Structural analysis showed that the structures of tree peony SWEET proteins were very conserved in the evolutionary process. The 10 peony SWEET proteins had 5 identical motiations and all contained two MtN3 saliva domains. (3) The results of soluble sugar content showed that the content of sucrose in tree peony petals decreased continuously, while the contents of fructose and glucose increased continuously, and both reached the peak in the blooming period. (4) Through the analysis of RT qPCR, we found that the expression level of PsSWEET4 at flowering stage was 34 times than that in small bell, and the expression level of PsSWEET8 at flowering stage was 60 times than that in small bells. Combined with the relationship between the expression and evolution of these two genes and the change value of soluble sugar, we presumed that PsSWEET4 and PsSWEET8 might regulate the flowering process indirectly by regulating the transport of fructose and glucose, which layed a foundation for further study of the function of peony SWEET genes in the plant growth and development.
keywords:tree peony  SWEET genes  bioinformatics  expression analysis
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