内源GSH参与低温胁迫下外源NO对黄瓜幼苗叶片光合作用的调控
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引用本文:索琳格,崔金霞,吴 佩,张文博,杨志峰,刘慧英.内源GSH参与低温胁迫下外源NO对黄瓜幼苗叶片光合作用的调控[J].西北植物学报,2019,39(12):2207~2217
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索琳格1,2,崔金霞1,2*,吴 佩1,2,张文博1,2,杨志峰1,2,刘慧英1,2 (1 石河子大学 农学院新疆石河子 8320002 特色果蔬栽培生理与种质资源利用兵团重点实验室新疆石河子 832000) 
基金项目:国家自然科学基金 (31560571);
中文摘要:为探讨还原型谷胱甘肽(GSH)参与低温(10 ℃/6 ℃)胁迫下一氧化氮(NO)对黄瓜幼苗叶片光合及荧光作用的调节作用,该研究以‘津研4号’黄瓜幼苗为试材,于四叶一心时置光周期 14 h /10 h、昼夜温度 25 ℃/20 ℃光照培养箱内,叶面预处理分别为:双蒸水(对照)、GSH 合成酶抑制剂(BSO,丁硫氨酸亚砜胺)、NADPH 合成酶抑制剂(6 AN,6 氨基烟酰胺),8 h 后再喷施NO 供体亚硝基铁氰化钠(SNP),于第 1 次喷施药剂24 h 后昼夜温度降至10 ℃/6 ℃,低温胁迫 24 h 后测定叶片细胞膜透性、气体交换参数、叶绿素荧光等各项指标。结果显示:(1)与CK相比,SNP处理显著提高了黄瓜幼苗叶片相对含水量、叶绿素含量、净光合速率(Pn)、蒸腾速率(Tr)、气孔导度(Gs)、最大光化学量子产量(Fv/Fm)、实际光化学效率(ΦPSⅡ)、非光化学猝灭系数(NPQ)、光化学猝灭系数(qP)、表观光合电子传递系数(ETR)及NO含量,显著降低叶片胞间CO2浓度(Ci)、电解质渗漏率、丙二醛(MDA)含量、非调节性能量耗散量子产量YNO。(2)SNP处理可保持较高的荧光产额,使快速叶绿素荧光诱导曲线JIP相逐渐升高,而BSO+SNP、6 AN+SNP处理显著降低了SNP的作用效果;SNP处理黄瓜幼苗叶片PSⅡ最大光化学效率(φPo)、PSⅡ反应中心吸收光能用于电子传递的量子产额(φEo)、捕获激子将电子传递到电子传递链QA-下游其他电子受体的概率(ψo)、与PSⅡ反应中心受体侧性能有关的荧光参数包括氧气释放复合体(OEC)活性状态(FO K)、QA被还原能力(FK J)、QB(含快还原PQ库)被还原能力(FJ I)和慢还原PQ库被还原能力(FI P)以及单位受光面积有活性反应中心数量(RC/CS)等均显著高于CK,而与反应中心关闭有关的荧光参数QA被还原最大速率(Mo)和J相相对可变荧光(VJ)均显著低于CK。(3)BSO+SNP、6 AN+SNP处理也削弱了SNP的这些作用效果。研究表明,在低温胁迫条件下,内源GSH参与了外源NO增强黄瓜幼苗叶片相对含水量、缓解膜脂过氧化、稳定PSⅡ和促进PSⅡ反应中心电子传递链供受体侧的电子传递能力,从而提高黄瓜幼苗耐冷性。
中文关键词:黄瓜幼苗  低温胁迫  一氧化氮(NO)  光合作用  生理特性
 
Endogenous GSH Involved in the Effect of Exogenous Nitric Oxide on Photosynthetic Characteristics of Cucumber Seedling Leaves under Low Temperature Stress
Abstract:The objective of this experiment was to investigate the reduced glutathione (GSH) participated in the effects of photosynthesis and fluorescence regulation pathway of exogenous nitric oxide (NO) in cucumber seedling leaves under low temperature (10 ℃/6 ℃) stress. The substrate cultured ‘Jinyan No.4’ cucumber seedlings were used as the test materials. Four leaf one hearted plants were set at a photoperiod of 14 h/10 h, day and night temperature of 25 ℃/20 ℃ in a light incubator, and leaf surface pretreatment with GSH synthase inhibitors (BSO, butanamine sulfoxide amine), NADPH synthetase inhibitor (6 AN, 6 amino nicotinamide), and nitric oxide NO donor (SNP, sodium nitroferrocyanide) after 8 hours The day night temperature dropped to 10 ℃/6 ℃ 24 h after the first spraying, and cucumber leaves cell membrane permeability, gas exchange and chlorophyll fluorescence parameters and other changes were measured after 24 h of cold stress. The results showed that: (1) compared with the control, the SNP treatment significantly increased the relative water content, chlorophyll content, net photosynthetic rate (Pn), transpiration rate (Tr) and stomatal conductance (Gs), Maximum photochemical efficiency (Fv/Fm) of photosystem Ⅱ (ΦPSⅡ), the quantum yield of PSⅡ regulated energy dissipation (NPQ), photochemical quenching coefficient (qP), apparent photosynthetic electron transport rate (ETR), the relative fluorescence of the nitric oxide, the OJIP phase of rapid chlorophyll fluorescence induction curve of cucumber seedlings and maintain high fluorescence yield after 24 h of low temperature stress, significantly decreased intercellular CO2 concentration (Ci), alleviated continual enhancement of MDA content and the quantum yield of non regulatory energy dissipation YNO. (2) Compared with SNP treatments, BSO+SNP and 6 AN+SNP treatments significantly reduced the effect of SNP; SNP treatment of cucumber seedling leaves the maximal photochemical efficiency of primary photochemistry (φPo), the quantum yield of light energy absorption by PSⅡ reaction center for electron transfer (φEo) and the probability of capturing excitons for transporting electrons to other electron acceptors downstream of the electron transport chain QA (ψo), fluorescence parameters related to the acceptor side properties of the PSⅡ reaction center include the oxygen release complex (FO K), the reduction capacity of QA (FK J), the reduction capacity of QB (FI P) and the density of active reaction centers per excited cross section (RC/CS) were significantly higher than that of CK. (3) Compared with SNP treatment, the values of φPo, φEo, ψo, RC/CS, FK J, FJ I and FI P were significantly decreased in BSO+SNP and 6 AN+SNP treatment, while Mo and VJ increased significantly. Under low temperature stress, GSH plays an important role in exogenous NO increasing the relative water content, reducing lipid peroxidation, stabilizing photosystem Ⅱ and promoting electron transfer capability of donor side and recipient side of electron transfer chain in PSⅡ reaction center and improved the cold resistance of cucumber seedlings.
keywords:cucumber seedling  low temperature stress  nitric oxide (NO)  photosynthetic characteristics  physiological characteristics
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