一株产虫草素的冬虫夏草内生真菌 Bionectria ochroleuca OS17
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引用本文:闵长莉,闵运江,汪学军.一株产虫草素的冬虫夏草内生真菌 Bionectria ochroleuca OS17[J].西北植物学报,2019,39(12):2271~2277
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作者单位
闵长莉1,2,闵运江1,2,汪学军1,2 (1 皖西学院 生物与制药工程学院安徽六安 237012
2 安徽省石斛产业化开发协同创新中心安徽六安 237012) 
基金项目:安徽省自然科学基金(1708085MH226);
中文摘要:虫草素是一种核苷类似物,具有多种药理作用。利用虫草素产生菌发酵的方式获得虫草素,可能是产生虫草素的一种新方法。为筛选能产虫草素的内生真菌,从中国传统的中药冬虫夏草子实体中共分离得到42株真菌,经PDB液体培养后,采用高效液相色谱(HPLC)鉴定其代谢产物。结果表明:菌株OS17的代谢产物中含有虫草素,且虫草素产量为40.16 mg/L。基于形态学特征和ITS序列分析,菌株OS17被鉴定为淡色生赤壳菌(Bionectria ochroleuca)。这是首次从冬虫夏草中分离得到能产虫草素的生赤壳属,表明了内生真菌具有产生多种生物活性物质的潜力。
中文关键词:淡色生赤壳菌  冬虫夏草  分离  虫草素
 
Bionectria ochroleuca OS17: An Endophytic Fungus from Ophiocordyceps sinensis That Produces Cordycepin
Abstract:Fermentation processes using cordycepin producing fungi other than O. sinensis may be an alternative way to produce cordycepin, which is a nucleoside analogue compound possessing many pharmacological functions. In order to screen cordycepin producing fungi from traditional Chinese medicine Ophiocordyceps sinensis, we isolated a total of 42 fungal strains from the fruiting bodies of O. sinensis. In the present study, a cordycepin producing fungal strain OS17 was isolated from O. sinensis. The strain produced cordycepin when grown in potato dextrose broth (PDB) medium, and the amount of cordycepin produced by this fungus was quantified to be 40.16 mg/L by HPLC. The fungal cordycepin which was analyzed by HPLC was shown to be identical to authentic cordycepin. The strain OS17 was identified as Bionectria ochroleuca based on its morphology and ITS sequence analysis. To the best of our knowledge, this is the first report concerning the isolation and identification of cordycepin producing Bionectria spp. from its host, which showed that endophytic fungi have the potential to produce many bioactive compounds.
keywords:Bionectria ochroleuca  Ophiocordyceps sinensis  isolation  cordycepin
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