Abstract:Plantspecific transcription factors NAM family belongs to NAC transcription factor superfamily that play an important role in plant growth and development, physiological metabolism and response to various stresses. This study used bioinformatics methods to identify NAM genes in rice genome, analyzed their spatiotemporal expression patterns, subcellular localization and protein interaction, and used realtime quantitative qRTPCR to analyze the expression characteristics of NAM genes under different exogenous hormones (such as SA, ABA and MeJA) and abiotic stresses (including drought, salt and cold), and laid the foundation for further exploring the function and stress mechanism of NAM genes in abiotic stress and hormone regulation pathways. The results showed: (1) 48 NAM genes were identified from rice genome, clustered into five subfamilies through evolutionary analysis. There were nine pairs segmentally duplications of NAM genes in the rice genome. (2) Tissue expression analysis showed that NAM genes specifically expressed in different tissues and developmental stages of rice, especially high expression in the leaf sheath, stems and nodes; most of them are nuclear localization; there are many protein interactions. (3) Realtime quantitative qRTPCR expression analysis showed that 10 NAM genes were specifically expressed in different tissues; most of NAM genes were upregulated under salt and drought stresses, while the expression was low under cold stress.; the transcript levels of NAM genes were highly altered under SA, ABA, and MeJA application. The results showed that NAM genes play an important role in rice growth and development, hormones response and abiotic stresses response.