黑胫病菌侵染过程中油菜响应基因的表达分析
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引用本文:郝丽芬,燕孟娇,房永雨,史志丹,李子钦,韩 冰.黑胫病菌侵染过程中油菜响应基因的表达分析[J].西北植物学报,2021,41(2):197~211
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作者单位
郝丽芬1,2,燕孟娇2,房永雨2,史志丹2,李子钦2,韩 冰1* (1 内蒙古农业大学 生命科学学院呼和浩特 0100112 内蒙古自治区农牧业科学院呼和浩特 010031) 
基金项目:国家重点研发计划(2018YFD0200907);
中文摘要:油菜黑胫病是造成油菜产量损失的病害之一,致病菌为Leptosphaeria biglobosa。该研究采用形态学观察和转录组测序技术,分析油菜接种病原菌Leptosphaeria biglobosa 4、12、24、36、48和96 h后的表型及基因表达变化情况,以探讨响应死体营养型真菌L. biglobosa侵染时油菜的防御反应及抗病机理,为揭示油菜与L. biglobosa互作的分子机制提供理论依据,并为培育油菜抗病品种积累了基因资源信息。结果显示:(1)接种4~96 h,叶片病斑逐渐扩大,病原菌侵染48~96 h后形成菌丝网。(2)通过RNA Seq测序,在L. biglobosa侵染油菜的不同时间点(4、12、24、36、48和96 h)分别得到3 384、2 270、3 802、5 811、6 155和7 153个差异表达基因。(3)15个油菜差异表达基因的qRT PCR检测表达水平与转录组测序结果基本一致。(4)利用短时间序列聚类和KEGG富集分析差异表达基因,结果发现植物病原菌互作、蛋白激酶、茉莉酸/乙烯/水杨酸和芥子油苷合成途径中的基因被强烈诱导表达,而且基因表达呈动态变化趋势。
中文关键词:差异表达基因(DEGs)  Leptosphaeria biglobosa  植物 病原菌互作  MAPK  茉莉酸/乙烯/水杨酸  芥子油苷合成
 
Gene Expression Analysis of Brassica napus in Response to Leptosphaeria biglobosa Infection Progress
Abstract:Blackleg caused by Leptosphaeria biglobosa, is one of the damaging diseases of oilseed rape (Brassica napus). Based on histological observation and RNA Seq, we analyzed the phenotypes and gene expression changes of oilseed rape after inoculation with L. biglobosa for 4, 12, 24, 36, 48 and 96 h to discuss the defense response and disease resistance mechanism of oilseed rape in response to the infection of L. biglobosa, provided a theoretical basis for comprehensively revealing the molecular mechanism of interaction between oilseed rape and L. biglobosa, and accumulated genetic information for breeding the disease resistance varieties of oilseed rape. The results showed that: (1) after inoculation for 4-96 h, the leaf spot gradually expanded, and the L. biglobosa formed mycelium within 48-96 h. (2) By RNA Seq, a number of 3 384, 2 270, 3 802, 5 811, 6 155, 7 153 differentially expressed genes (DEGs) were obtained at 4, 12, 24, 36, 48 and 96 h of L. biglobosa infection on oilseed rape. (3) qRT PCR results showed that the levels of the 15 differentially expressed genes in oilseed rape were basically consistent with the results of RNA Seq. (4) The differentially expressed genes were analyzed by short time series clustering and KEGG enrichment analysis. The results showed that the genes in the pathways of plant and L. biglobosa interaction, protein kinase, jasmonate/ethylene/salicylic acid signaling, and glucosinolate biosynthesis were strongly activated, and their gene expression showed dynamic change trend.
keywords:differentially expressed genes (DEGs)  Leptosphaeria biglobosa  plant pathogen interaction  MAPK  jasmonate/ethylene/salicylic acid  glucosinolates biosynthesis
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