基于转录组和代谢组分析马齿苋根茎叶中类黄酮代谢
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引用本文:张少平,邱珊莲,黄惠明,张 帅,林宝妹,洪佳敏,郑开斌.基于转录组和代谢组分析马齿苋根茎叶中类黄酮代谢[J].西北植物学报,2021,41(2):254~261
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张少平,邱珊莲,黄惠明,张 帅,林宝妹,洪佳敏,郑开斌* (福建省农业科学院 亚热带农业研究所福建漳州 363005) 
基金项目:福建省农业科学院创新团队项目(STIT2017 2 11);
中文摘要:为获取马齿苋(Portulaca oleracea)类黄酮相关成分及其合成酶基因信息,该实验以马齿苋根、茎和叶为材料,进行代谢组学和转录组学联合分析,并从中选取6个差异表达基因进行qRT PCR验证分析。结果显示:(1)代谢组分析发现,在马齿苋根、茎和叶中共获得32个类黄酮相关化合物,包括3个异黄酮、8个黄酮醇、11个黄酮、3个黄烷酮、5个黄烷醇和2个花青素,其中20种类黄酮化合物在马齿苋根、茎和叶部含量接近,而另外12种在不同部位含量差异明显。(2)转录组分析发现,在马齿苋根、茎和叶中共获得93条类黄酮主要合成酶基因簇信息,包括20条CHS、3条CHI、7条F3H、2条ANS、11条IFS、21条F3′H、2条F3′5′H、2条DFR、2条ANR、1条LAR和22条UF3GT。(3)qRT PCR结果显示,所获取的6个参与类黄酮合成的酶基因,在马齿苋根、茎和叶中上下调表达趋势与转录组测序结果完全一致,但不同合成酶基因差异表达倍数不完全相同。研究表明,在马齿苋根、茎和叶中存在大量的类黄酮化合物及其合成酶基因,但不同类黄酮成分在不同部位含量不同,其合成代谢相关酶在不同部位的表达量也存在差异。
中文关键词:马齿苋  转录组  代谢组  类黄酮成分  合成酶
 
Transcriptome and Metabolome Analyses of Flavonoids Metabolism in Leaf, Stem and Root of Portulaca oleracea L.
Abstract:In order to explore relevant information of flavonoid componts and their synthases in Portulaca oleracea L., we utilized both the transcriptome and metabolome to analyze the root, stem and leaf of P. oleracea L. At the same time, 6 differential expressed genes were identified by the RT PCR. The results showed that: (1) by the metabolome, there were 32 flavonoid components included 3 isoflavones, 8 flavonols, 11 flavones, 3 flavanones, 5 flavanols and 2 canthocyanidins in which the contents of 20 flavonoid components were similar, while the other 12 ones were significantly different in root, stem and leaf of P. oleracea L. (2) By the transcriptome, there were 93 flavonoid synthases which included 20 CHSs, 3 CHIs, 7 F3Hs, 2 ANSs, 11 IFSs, 21 F3′Hs, 2 F3′5′Hs, 2 DFRs, 2 ANRs, 1 LAR and 22 UF3GTs in root, stem and leaf of P. oleracea L. (3) According to the result of transcriptome, the expressed trend of these 6 flavonoid synthases agreed with the testing by the RT PCR. However, the trend multiples of these differentially expressed genes by these 2 kinds of approaches were different. So this study concluded that there are many flavonoid components and their synthases in P. oleracea L. including root, stem and leaf, but the contents of flavonoids and the expression of their enzymes in different tissues are distinct.
keywords:Portulaca oleracea  transcriptome  metabolome  flavonoid component  synthase
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