滇南农家大麻品种中大麻素化学型及基因型研究
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引用本文:陈 璇,郭孟璧,张庆滢,许艳萍,郭鸿彦,杨 明,杨清辉.滇南农家大麻品种中大麻素化学型及基因型研究[J].西北植物学报,2013,33(9):1817~1822
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作者单位
陈 璇1,2,郭孟璧2,张庆滢2,许艳萍2,郭鸿彦2,杨 明2,杨清辉1* (1 云南农业大学 农学与生物技术学院,昆明 650201
2 云南省农业科学院经济作物研究所,昆明 650205) 
基金项目:国家自然科学基金(31160296);云南省科技计划(2012FB190);云南省科技计划重点新产品开发项目(2011BB011)
中文摘要:以1个滇南农家大麻品种群体为研究对象,通过化学分析及同源克隆方法,研究了21个单株中2种主要大麻素——四氢大麻酚(THC)和大麻二酚(CBD)的化学型和基因型,以揭示大麻素含量、化学型以及基因型三者之间的关系,为工业大麻新品种选育提供理论依据。研究表明:(1)化学检测结果显示,21个单株均含有THC,THC含量在0.07%~1.35%之间,其中7个单株仅含THC,5个单株含THC和微量CBD,9个单株同时含有THC和CBD,CBD含量范围为0~0.58%。(2)CBD/THC比值显示,该群体仅存在毒品型和中间型2种化学型,且中间型大麻中THC和CBD含量显著正相关。(3)基因扩增及测序分析结果显示,该群体为基因型杂合群体,群体内THCA合成酶基因存在5个变异位点,CBDA合成酶基因存在2个变异位点,但变异位点和THC及CBD的含量直接关系。(4)群体内单株的基因型和化学型完全对应,且THCA合成酶基因及CBDA合成酶基因可作为分子标记来鉴定单株化学型。
中文关键词:大麻  大麻素  四氢大麻酚  大麻二酚  化学型  基因型
 
Chemotype and Genotype of Cannabinoids in Hemp Landrace from Southern Yunnan
Abstract:A hemp landrace from southern Yunnan was selected to study the chemotype and genotype of two main cannabinoids (tetrahydrocannabinol,THC;cannabidiol,CBD) by chemical test and gene cloning,respectively.The results are:(1)Chemical test revealed that all 21 plants contained THC,and the THC content ranged from 0.07% to 1.35%.In this population,9 plants contained THC alone,5 plants contained THC and trace CBD,and the other 9 plants included both THC and CBD,while the CBD content ranged from 0 to 0.58%.(2)Accordingly to the content ratio of CBD/THC,only drug-type and intermediate-type were existed in the landrace.There was also a positive correlation between THC and CBD contents in the plants of intermediate-type.(3)Gene amplification and sequence alignment showed 5 base variations and 2 base variations in THCA synthase genes and CBDA synthase genes,respectively,and the variations had no direct relations with the contents of THC and CBD.(4)The genotype completely matched with chemotype in 21 plants,and the two synthase genes can be made as molecular marker to identified chemotype in a single plant.
keywords:Hemp  cannabinoid  THC  CBD  chemotype  genotype
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