硬粒小麦-长穗偃麦草2E和4E附加系及E染色体传递
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1.扬州大学生物科学与技术学院;2.扬州大学生科院

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江苏省自然科学基金(编号:BK20180908;BK20181213);扬州市自然科学基金(编号:YZ2018097)


Durum-Thinopyrum elongatum 2E and 4E addition lines and E chromosome transmission
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[Supported by The Natural Science Foundation of Jiangsu(No. BK20180908;BK20181213); The Natural Science Project of Yangzhou(No. YZ2018097)]

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    摘要:

    为探讨长穗偃麦草E染色体在硬粒小麦背景中的传递特点,利用染色体特异分子标记、基因组原位杂交(GISH)、非变性荧光原位杂交(ND-FISH)等方法,对小偃麦8801(AABBEE)与硬粒小麦(AABB)杂交后代中选育的株系Du_No.2和Du_No.4进行了分析。分子标记检测株系Du_No.2及Du_No.4分别能扩增出长穗偃麦草2E、4E染色体特异条带。GISH和ND-FISH结果表明株系Du_No.2和Du_No.4分别附加了一条2E和4E染色体。2个株系的减数分裂显示,后期Ⅰ、Ⅱ和末期Ⅱ都有E染色体分离异常现象。另外,2个株系与硬粒小麦的正反交表明2E和4E染色体在硬粒小麦背景中能通过雄配子传递,但未见通过雌配子传递。本研究为创建全套硬粒小麦-长穗偃麦草双体附加系及代换系提供基础。

    Abstract:

    To explore the transmission characters of chromosomes from Thinopyrum elongatum in the background of durum, The lines Du_No.2 and Du_No.4 derived from a cross between Tritileytrigia 8801(AABBEE) and T. turgidum L. ssp. durum(AABB) were characterized by chromosome-specific molecular markers, genomic in situ hybridization(GISH), and non-denaturing fluorescence in situ hybridization(ND-FISH). The results of molecular markers indicated that lines Du_No.2 and Du_No.4 could respectively amplify the specific band of 2E,4E chromosome from Th. elongatum. The results of GISH and ND-FISH showed that lines Du_No.2 and Du_No.4 had a 2E and 4E chromosomes of Th. elongatum respectively. The results of meiosis indicated that both lines have E chromosome segregation abnormalities in anaphase I, II and telophase II of meiosis. In addition, the positive and negative crosses between the two lines and durum displayed that chromosomes 2E and 4E could be transmitted through male gametes in durum background, but could not be transmitted through female gametes. This study provided a basis for the establishment of a complete set of durum-Th. elongatum addition lines and substitution lines.

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  • 收稿日期:2019-03-28
  • 最后修改日期:2019-05-10
  • 录用日期:2019-05-13
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