Abstract:In this study,based on the transcriptome results of jasmine petals, the full length cDNA and promoter sequence of NEL/LINS were cloned using RT-PCR and Genome Walking Technique, respectively. The content of linalool was detected by GC-MS, and the expression level of JsNEL/LINS during the opening process and after treatment of IAA, GA, ABA and MeJA. The results showed that the full length cDNA of JsNEL/LINS (GenBank accession number:MH513857) was 1843bp,contained 1755 bp open reading frame (ORF),encoding a protein of 584 amino acids, and belonged to hydrophilic and stable protein. The results of NCBI-BLAST showed that JsNES/LINS had 56%,54%,54% and 50% similarities with Olea europaea OeNES,Antirrhinum majus AmNES/LINS1,AmNES/LINS 2 and Camellia saluenensis CsLINS,respectively. The result of Subcellular localization showed that JsNEL/LINS was located on the cytoplasm. 1141bp promoter sequence of the JsNES/LINS was isolated, and the result predicted that it contained several cis-acting elements involved in plant hormones and light response,indicating that the expression of JsNAL/LINS was regulated by plant hormones and light. The results of GC-MS analysis showed that the relative content of linalool was the lowest when jasmine was not opened at 18:00, and reached the maximum when it was half open at 22:00, and then decreased. The analysis of RT-qPCR showed that the relative expression level of JsNES/LINS was consistent with the change of the relative content of linalool compounds during the flowering process. The expression of JsNES/LINS was induced by IAA, GA, ABA and MeJA, and the induction of MeJA was the most obvious, and the induction from high to low were MeJA>GA>ABA>IAA. It is speculated that JsNES/LINS plays an important role in the biosynthesis of linalool compounds and is regulated by plant hormones.