StMYB44基因参与马铃薯对蔗糖胁迫的响应
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1.青海大学;2.青海省农林科学院

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现代农业产业技术体系(CARS-9);专用型马铃薯产业高质量发展关键技术研发与示范(2019-NK-A1)


StMYB44 is Involved in Response to Sucrose Stress in Potato
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    摘要:

    MYB转录因子存在于所有真核生物中,是最具代表的转录因子家族之一,广泛参与植物发育、苯丙烷代谢、生物与非生物胁迫响应、激素响应以及细胞形态建成等过程。本研究以马铃薯青薯9号为实验材料克隆了StMYB44基因(GenBank No.XM_006367359.2),该基因的CDS长度为963bp,编码320个氨基酸,含有2个SANT结构域。实时荧光定量PCR结果表明,StMYB44在花中表达最高,在匍匐茎中表达最低;StMYB44在无蔗糖处理下表达上调,在高浓度蔗糖处理下(6%、9%)表达下调。构建表达载体并遗传转化烟草发现,StMYB44突变体在无蔗糖条件下的长势明显好于野生型,而在高蔗糖浓度下(6%、9%)的长势弱于野生型,并且蔗糖浓度越高,差异越大。这些结果说明蔗糖浓度能够调节StMYB44的表达,推测该基因可能在植物响应蔗糖中起重要作用。

    Abstract:

    MYB transcription factor is one of the most representative transcription factor families in all eukaryotes. It is widely involved in plant development, phenylpropanoid metabolism, biotic and abiotic stress response, hormone response, and cell morphogenesis. In this study, StMYB44 gene (GenBank No.XM_006367359.2) was cloned from potato, whose sequence length was 963 bp, encoded 320 amino acids that contained two SANT domains. The result of quantitative real-time PCR showed that StMYB44 had the highest expression in flowers and the lowest expression in stolon; StMYB44 was up-regulated under sucrose-free treatment and down-regulated under high-concentration sucrose treatment (6%, 9%). Construction of expression vector and genetic transformation of tobacco found that the growth of StMYB44 mutant under sucrose-free conditions was significantly better than that of wild-type, while at high sucrose concentration (6%, 9%), the growth was weaker than wild-type, and the higher the sucrose concentration, the difference is greater. These results indicates that sucrose concentration can regulate the expression of StMYB44, suggesting that this gene may play an important role in plant response to sucrose.

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  • 收稿日期:2019-11-21
  • 最后修改日期:2020-01-10
  • 录用日期:2020-01-10
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