‘砀山酥梨’黄化叶复绿过程中内源IAA含量及其信号传导基因表达量分析
DOI:
作者:
作者单位:

安徽农业大学园艺学院

作者简介:

通讯作者:

中图分类号:

基金项目:

国家现代农业产业技术体系建设专项(CARS-29-14);梨树和桃树化肥减施增效关键技术与产品研发(2018YFD0201401)


Analysis of Endogenous IAA Content and Signaling Genes Expression in Retrieved leaves of ‘Dangshansuli’ Pear (Pyrus bretschneideri Rehd.)
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    为探讨IAA其在梨黄化叶复绿过程的作用,以‘砀山酥梨’(Pyrus bretschneideri Rehd.)黄化植株与正常植株为试材,对生长期黄化植株叶面喷施0.20% FeSO4溶液。在处理后第3、56、79、12天分别观察黄化叶复绿情况,取样测定黄化叶片复绿过程中各处理时期叶片和对照组正常、黄化叶片Fe2+含量采用ELISA酶联免疫法,测定叶片中、内源IAA含量;并参考NCBI、梨基因组等信息,查询了IAA相关信号转导基因,运用qRT-PCR技术,分析、IAA相关信号转导基因其在黄化叶片复绿过程中的相对表达量,并分析Fe2+、IAA含量及相关基因表达量之间相关性。结果表明:(1)FeSO4溶液处理可致使黄化叶片复绿。黄化叶面在处理后第3 天开始出现复绿斑点,在处理第6、9天时出现大范围的复绿斑块,在第12 d天出现整叶复绿。(2)ELISA测定结果表明,对照组正常叶内源IAA含量显著高于对照组黄化叶,FeSO4处理后各个时期的复绿叶内IAA含量均较黄化叶增加,且显著高于对照组正常叶和黄化叶。(3)qRT-PCR结果表明,对照组黄化叶内TIR1.1、AUX1.1/1.3、GH3.1/3.2/3.5、SAUR1/2/3/4、AUX1.2/1.3、AUX/IAA1/32/73/77、SAUR1/2/3/4表达量均显著高于正常叶,在FeSO4处理后其表达量大多较黄化叶显著减少;而对照组黄化叶内TIR1.1、GH3.3/3.4、AUX/IAA4/5/6表达量量均显著低于正常叶,在FeSO4处理后其各时期表达量均较黄化叶显著增加。(4)对照组黄化叶内ARF3/4/5/6/7/8/9/10/15/17/ 18/19/20/21/22/23表达量均显著低于正常叶,FeSO4处理显著促进了ARF3/5/6/9/19/20/21/22/23的表达;而对照组黄化叶内ARF1/2/11/12/13/14表达量显著高于正常叶,FeSO4处理则显著抑制了ARF1/2的表达。(5)相关性分析结果表明,FeSO4处理后复绿叶内显著增加的Fe2+含量主要与AUX/IAA5、AUX/IAA6表达量显著上调呈正相关;而IAA含量的显著增加则与GH3.3表达量显著上调呈正相关,、与SAUR1/2负相关表达量显著下调相关。两者之间相关网络图错综复杂,其简要途径为:Fe2+→AUX/IAA5、AUX/IAA6→(ARF14)→ ARF5→ SAUR1、AUX/IAA1→SAUR2/TIR1.1/GH3.3→IAA。 研究认为,IAA及其信号转导基因在FeSO4所诱导的‘砀山酥梨’缺铁黄化叶复绿过程中可能发挥重要作用。

    Abstract:

    To reveal the role of IAA and its signaling genes in the retrieved green process of pear leaves, chlorotic and normal plants of ‘Dangshansuli’ (Pyrus bretschneideri Rehd.) were taken as test material. What’s more, 0.20% FeSO4 solution were sprayed on the chlorotic leaves and distilled water were used for both normal and chlorotic leaves as control during the growing season, . then Then observing the leaves phenotypic changes on 3rd, 6th, 9th, and 12th d after FeSO4 application, respectively..Ssampling normal, chlorotic leaves in control (CN and CC)andthe leaves of retrieved green leaves (RL) on 3rd, 6th, 9th, 12th d after the 0.20% FeSO4 application and storing them in ?80℃ refrigerators, to determine . Tthe Fe2+ content, endogenous IAA content and of samples were determined by ELISA and the expression of IAA signaling genes expression related to IAA signal transduction were analyzed by qRT-PCR with reference to the NCBI and pear genome. Furthermore, relevance analysis was performed by SPSS among those indexes. The results were shown as followingfollows: (1) Application FeSO4 could convert chlorotic leaves to green, Green green spots appeared on the chlorotic leaf leaves surface on 3rd d, and green plaque appeared on both 6th and 9th d after spraying FeSO4. What’s more, the whole leaf presented green on 12th d. (2) The endogenous IAA content in CNnormal leaves of control (NL) was significantly higher than that in CCchlorotic leaves (CL), and after the sprayingapplication of FeSO4, it obviously increased in RL retrieved green leaves compared to with both CN and CC CL in all periods, which were significantly higher than NL. (3) The expression of AUX1.1/1.3、GH3.1/3.2/3.5、SAUR1/2/3/4 and AUX/IAA1/2/3/7TIR1.1, GH3.1/3.2/3.5, AUX1.2/1.3, AUX/IAA1/3/7 and SAUR1/2/3/4 expression in CC CL were prominently higher than that in CN NL, and FeSO4 applicationmost of them were notably inhibited their expression in an outstanding levelin all period after spraying FeSO4. In contrastMeanwhile, TIR1.1、GH3.3/3.4 and AUX/IAA4/5/6 and GH3.3/3.4 expression in CcCL were distinctively lower than that in CNNL, and they were all apparently promoted by FeSO4 application. (4) ARF3/4/5/6/7/8/9/ 10/15/17/18/19/20/21/22/23 in Cc were clearly decreased compared with CN, and FeSO4 application significantly promoted the expression of ARF3/5/6/9/19/20/21/22/23; On the contrary, ARF1/2/11/12/13/14 were remarkably increased in CcCL compared to CNNL, and FeSO4 application remarkably suppressed the expression of ARF1/2. (5) The relevance analysis showed that remarkably increased Fe2+ content in retrieved green leaves was positively correlated with to the up-regulation of AUX/IAA5 and AUX/IAA6 expression, while IAA content was positively correlated with the increased GH3.3 expression and negatively correlated with the decreased SAUR1/2 expression. The network diagram of the association between the two above is complex, which can be simplified into Fe2+→ AUX/IAA5, AUX/IAA6→(ARF14)→ ARF5 SAUR1, AUX/IAA1→ SAUR2, TIR1.1, GH3.3→ IAA. The study suggested that IAA and its signaling pathway might play a crucial role in the retrieved green process induced by spraying FeSO4 inon iron-deficiency chlorotic leaves of ‘Dangshansuli’.

    参考文献
    相似文献
    引证文献
引用本文
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2020-12-01
  • 最后修改日期:2021-04-10
  • 录用日期:2021-04-01
  • 在线发布日期:
  • 出版日期:

微信公众号二维码

手机版网站二维码