欧李果实酚类物质及抗氧化活性特征研究
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内蒙古农业大学 园艺与植物保护学院

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内蒙古科技成果转化专项(2019CG067)、新品种选育专项(YZGC2017019)、内蒙古自治区直属高校基本科研业务费项目共同资助。


Study on Phenolic Content and Antioxidant Activity in Fruits of Different Cerasus humilis Resource
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    摘要:

    以内蒙古地区36种欧李优系资源果实为试验材料,比较分析了果实酚类物质含量与抗氧化活性的特征差异、资源变化特点及两者之间的关系;并分别以酚类物质含量及抗氧化活性指标为参数对欧李资源进行了聚类分析及评价。结果表明:⑴不同资源类型欧李果实的总酚、总黄酮、原花青素含量和抗氧化活性均表现出明显的差异。⑵不同果肉颜色和不同酸度的欧李果实酚类物质含量均表现不同,红色和桔黄果肉的果实以上3种酚类物质含量整体上均高于浅红果肉的果实;酸度<1.60%时,果实3种酚类物质含量均明显高于酸度为1.60%-1.70%及>1.70%的果实。⑶分别以3种酚类物质含量及4项抗氧化活性指标为参数,利用SPPS26.0软件对欧李资源进行聚类,将欧李资源分别划分为高酚、中酚及低酚3种类型及高、中、低3种抗氧化活性类型,高、中、低抗氧化活性类型主要由相应的高酚、中酚及低酚的欧李资源组成;同时相关分析表明,3种酚类物质与总还原力、?OH清除率、DPPH?清除率多表现出极显著正相关(P<0.01)。研究发现,总酚、总黄酮及原花青素是构成欧李果实抗氧化活性的重要组成部分,3种酚类物质含量水平体现了果实抗氧化活性的强弱;经聚类分析筛选出高酚及高抗氧化活性欧李优系资源各6种,为进一步研究与应用提供基础材料。

    Abstract:

    The characteristics and variation of phenolic content and antioxidant activity, the relationship between them were compared and analyzed in fruits of 36 C .humilis superior lines resources in Inner Mongolia. Furtherly the phenolic content and antioxidant activity indexes were used as parameters to cluster and evaluate the resources of C .humilis. The results show that:⑴The contents of total phenols, total flavonoids, proanthocyanidins and antioxidant activities in fruits of different C .humilis resources showed obvious differences.⑵The phenolic content in C .humilis fruits with different flesh colors and acidity were different. The contents of the above three phenolic content in C .humilis fruits with red and orange flesh were higher than those with light red flesh. When acidity was less than 1.60%, the contents of three phenolic contentin in fruits were significantly higher than those in fruits with acidity of 1.60%-1.70% and >1.70%.⑶By clustering C .humilis resources with SPPS26.0 software as three phenolics content and antioxidant activity for the parameters respectively, C .humilis resources were divided into three phenolic content level types of high,medium, low and three antioxidant activity types of high,medium, low. Furtherfore, the C .humilis resources of three antioxidant activity types were consisted of corresponding-rank resources of three phenolic content level types.Correlation analysis also showed that the three phenolic content were positively correlated with total reducing power, ?OH scavenging rate and DPPH? scavenging rate (P<0.01). In conclusion ,total phenols, total flavonoids and proanthocyanidins were important components of the antioxidant activity of C .humilis fruits. The content level of the three phenolic reflected the strength of the antioxidant activity of C .humilis fruits. Six C .humilis superior lines resources with high phenol and six kinds of resources with high antioxidant activity were selected by cluster analysis, which provided basic materials for further research and application.

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邵郅胜,张薇,孙聪,等.欧李果实酚类物质及抗氧化活性特征研究[J].西北植物学报,2023,43(8):1405-1414

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  • 收稿日期:2022-10-28
  • 最后修改日期:2023-01-18
  • 录用日期:2023-07-05
  • 在线发布日期: 2023-09-08
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