摘要 为了探索盐胁迫对杠柳幼苗生长及次生代谢物的影响，初步了解杠柳盐耐受范围及盐胁迫对杠柳次生代谢产物的诱导作用。本试验以培养30 d的杠柳实生苗为材料，测定不同盐浓度（0（CK）、0.2 %、0.5 %、1.0 %、2.0 %）处理后，杠柳幼苗的生物量及总甾体成分、强心苷类成分（杠柳毒苷、杠柳次苷及杠柳苷元）、4-甲氧基水杨醛含量的变化特征。结果表明，杠柳幼苗在中低盐浓度盐胁迫下生长良好，且地上部分干鲜重及地下部分干重均有不同程度的增加，其干湿比也均随盐胁迫程度的增加逐渐升高；植株体内的主要有效成分如总甾体、强心苷类的含量在中等盐胁迫浓度作用下呈现明显上升趋势，而4-甲氧基水杨醛则在高盐胁迫下含量显著增加。研究发现，杠柳具有较高的耐盐性，且一定范围内的盐胁迫可显著提高杠柳幼苗根茎中主要药效成分含量。
Abstract In order to explore the effects of salt stress on the growth and secondary metabolites of Periploca sepium seedlings, preliminarily understand the salt tolerance range of Periploca sepium and the induction effect of salt stress on secondary metabolites of Periploca sepium. This experiment was conducted on the seedlings of Periploca sepium cultivated for 30 days to determine the changes in biomass, total steroids components, cardiac glycosides (periplocin,periplocymarin and periplogenin), and 4-methoxy salicylaldehyde content of Periploca sepium seedlings treated with different salt concentrations (0 (CK), 0.2%, 0.5%, 1.0%, 2.0%). The results showed that the Periploca sepium seedlings grew well under low concentrations of salt stress, and the dry and fresh weight of the aboveground and underground parts increased to varying degrees. Their dry to wet ratio also gradually increased with the increase of salt stress level; The content of the main effective components in the plant, such as total steroids and cardiac glycosides, showed a significant upward trend under moderate salt stress concentration, while the content of 4-methoxy salicylaldehyde significantly increased under high concentrations of salt stress. Research has found that Periploca sepium has high salt tolerance, and salt stress within a certain range can significantly increase the content of main pharmacological secondary metabolites in the roots and stems of Periploca sepium seedlings.