陆地棉GhCSN6A基因克隆及低磷胁迫下的表达
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国家自然科学基金项目(31101184)


Cloning of GhCSN6A Gene from Gossypium hirsutum L. and Its Expression under Low Phosphorus Stress
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    摘要:

    该研究基于陆地棉根部低磷胁迫基因表达谱芯片差异表达序列结果及基因组数据库,对表达差异序列ES816317进行克隆,利用生物信息学分析其核苷酸及蛋白序列,并通过qRT -PCR技术检测其组织表达模式和在低磷胁迫下的相对表达特征,为解析棉花GhCSN6A 的生物学功能奠定基础,并为棉花磷高效基因工程育种提供基因资源。结果表明:(1)成功获得陆地棉GhCSN6A基因,该基因的开放阅读框全长为948 bp,编码315个氨基酸; GhCSN6A蛋白为COP9信号小体复合亚基6a,属于MOV34蛋白超家族,具有MPN_CSN6结构域,定位于细胞核。(2)序列比对和进化分析显示,陆地棉GhCSN6A与木槿HsCSN6A、拟南芥AtCSN6A的相似性分别为95.87%和84.54%。(3)qRT-PCR分析表明,GhCSN6A基因在陆地棉的根、茎、叶、花中均有表达,且在叶中表达水平最高,但叶与根中的表达无显著差异;GhCSN6A基因在低磷处理24 h时根中相对表达量最低,但低磷处理72 h时根中的相对表达量最高达到了适磷(对照)处理的2倍。研究推测,陆地棉GhCSN6A基因在棉花响应低磷胁迫过程中具有重要作用。

    Abstract:

    In this study, the differentially expressed sequence ES816317 was cloned based on the differential expression sequence results of the low phosphorus stress gene expression profile chip in the roots of Gossypium hirsutum L. and the genome database. We used bioinformatics methods to analyze its nucleotide and protein. Its tissue expression pattern and relative expression under low phosphorus stress were detected by using qRT-PCR technology, so as to lay the foundation for analyzing the biological function of GhCSN6A in G. hirsutum and provide genetic resources for cotton phosphorus efficient genetic engineering breeding. The results showed that: (1) GhCSN6A gene of G. hirsutum L. was successfully cloned, and the full length of the open reading frame of the gene was 948 bp, encoding 315 amino acids. GhCSN6A protein, called COP9 signalosome complex subunit 6a, belonged to the MOV34 protein superfamily, had an MPN_CSN6 domain, and was localized in the nucleus. (2) Sequence alignment and evolution analysis showed that the similarity of GhCSN6A to HsCSN6A and AtCSN6A was 95.87% and 84.54%, respectively, so the gene was named GhCSN6A. (3) qRT-PCR analysis showed that GhCSN6A was expressed in all tested tissue including root, stem, leaf and flower, and the expression level was the highest in leaf, but there was no significant difference between leaf and root. The relative expression of GhCSN6A gene was the lowest in the root treated with low phosphorus for 24 h. However, the highest in the root treated with low phosphorus for 72 h, which was twice that of the suitable phosphorus (control) treatment. The study has speculated that GhCSN6A gene played an important role in the response to low phosphorus stress in G. hirsutum.

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卿桂霞,耿翡翡,梁颖颖,等.陆地棉GhCSN6A基因克隆及低磷胁迫下的表达[J].西北植物学报,2023,43(1):29-36

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  • 在线发布日期: 2023-02-17
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