糜子黄、黑种皮遗传及转录组学分析
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内蒙古农业大学农学院

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内蒙古自治区科技计划项目(2021GG0319)、国家自然科学基金项目(32060509)


Genetic and transcriptomic analysis of yellow and black seed coat color in pros millet
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    摘要:

    为了解糜子黑色种皮与黄色种皮的遗传规律以及其形成机理,利用黄色种皮品种‘黄粒糜I’和黑色种皮品种‘2016106I’构建杂种F1、F2代,分析种皮颜色的遗传规律,利用F3代籽粒进行转录组测序,挖掘影响糜子籽粒颜色的关键基因,结果表明:(1)通过对杂交组合黄粒糜Ⅰ×2016106I杂交组合的F1代和F2代群体籽粒的种皮颜色遗传分析,结果表明,黑色对黄色为显性,由单基因控制。(2)根据GO和KEGG富集分析结果,共筛选出13个与类黄酮合成途径相关的差异基因,仅有C2845_PM02G08740、C2845_PM04G29280和C2845_PM09G22680为上调表达基因,其余10个均为下调表达基因,这些基因编码肉桂醇脱氢酶和肉桂酰CoA还原酶等多种酶,与种皮颜色的形成密切相关,但与糜子种皮颜色形成相关的主效基因需要进一步研究才可确定。本研究为明确糜子种皮颜色形成的机理和糜子新品种的选育提供理论基础。

    Abstract:

    In order to understand the genetic law and formation mechanism of black and yellow prosomillet seeds, this study used yellow seed coat variety "Huang limi I" and black seed coat variety "2016106I" to construct F2 generation population, analyze the genetic law of seed coat color, use F3 generation seeds for transcriptome sequencing, and explore the key genes that affect the color of millet seeds. The results showed that: (1) Through the hybrid combination of yellow seed millet I × Genetic analysis of seed coat color of F1 and F2 populations of 2016106I hybrid combination showed that black was dominant to yellow and was controlled by a single gene. (2) According to the results of GO and KEGG enrichment analysis, a total of 13 differentially expressed genes related to flavonoid synthesis pathway were screened, only C2845_ PM02G08740、C2845_ PM04G29280 and C2845_ PM09G22680 is an up-regulated gene, and the other 10 are down-regulated genes. Among them, C2845 is the gene with the largest differential multiple_ PM04G29280, the multiple of difference is 4.88 times; These genes encode many enzymes such as cinnamyl alcohol dehydrogenase and cinnamyl CoA reductase, which are closely related to the formation of seed coat color. However, the major genes related to the color formation of the seed coat of millet can be determined only after further study. This study provides a theoretical basis for clarifying the mechanism of color formation of seed coat and breeding of new varieties of prosomillet.

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李强,白璐,高志军,等.糜子黄、黑种皮遗传及转录组学分析[J].西北植物学报,2023,43(8):1268-1275

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  • 收稿日期:2023-02-28
  • 最后修改日期:2023-05-21
  • 录用日期:2023-05-25
  • 在线发布日期: 2023-09-08
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