南京椴花化学成分鉴定及不同发育时期黄酮类化合物差异分析
作者:
作者单位:

1.南京中医药大学;2.sup>3.江苏省中国科学院植物研究所&/sup&;4.&

作者简介:

通讯作者:

中图分类号:

Q946.8

基金项目:

国家自然科学基金项目 31700477“基于低拷贝基因的椴树属系统发育及生物地理研究”;江苏省农业科技自主创新资金项目CX(22)3177“椴树属特色种质资源挖掘、评价及繁育技术集成”;南京市科技发展计划项目 2022CX56“银毛椴‘斯特林’选育及应用示范”;南京市绿化园林局科技项目合同 YLKJ202214JH “优良乡土树种糯米椴资源挖掘及繁育技术研究”。


Identification of Chemical Composition and Metabolic Analysis of Flavonoids in Flowers of Tilia miqueliana Maxim.
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    【目的】研究南京椴花化学成分及其主要成分黄酮类化合物在不同发育时期的变化规律,为后续采用多组学手段探究椴树花中重要药用化合物的代谢通路及调控机制解析奠定基础,同时为椴树花的采收提供理论依据。【方法】以10年生南京椴为试验材料,利用超高效液相色谱串联质谱(UPLC-MS/MS)技术分析南京椴花中代谢物组分及类黄酮含量差异。【结果】结果表明:(1)南京椴花中共鉴定得到46种化合物,其中有机酸及其衍生物13种、香豆素及其衍生物4种、酯1种、类黄酮28种。(2)盛花期是南京椴花中代谢物发生显著变化的分界点,与蕾期相比,开花期间代谢物变化更明显。(3)多重比较分析显示,26种黄酮类化合物在不同时期花中含量差异显著(P<0.05),阿福豆苷、山奈苷、槲皮苷、橙皮苷、花旗松素和芹菜素-7-O-葡萄糖醛酸酸在盛花期相对含量较高,原花青素A2和3种原花青素三聚体在末蕾期含量较高。【结论】研究认为,发育状态可作为判断南京椴花中次级代谢物含量的重要因素,末蕾期的标志性化合物是原花青素A2,盛花期的标志性化合物是芹菜素-7-O-葡萄糖醛酸,末蕾期和盛花期均具有采收价值。

    Abstract:

    【Objective】 To lay a foundation for the subsequent use of multi-omics methods to explore the metabolic pathways and regulatory mechanisms of important medicinal compounds of Tilia miqueliana Maxim., and provide a theoretical basis for the harvesting of flowers, the chemical composition of T. miqueliana flowers and the content changes of its signature components, flavonoids, in different growing stages were investigated. 【Methods】 The 10-year-old T. miqueliana was used as the test material, this experiment conducted to explore chemical components of flower and flavonoid content differences using ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). 【Results】 The results showed that, (1) A total of 46 compounds were identified, including 13 organic acids and their derivatives, 4 coumarins and their derivatives, 1 ester, and 28 flavonoids. (2) The flower opening stage was the cut-off point for significant changes in metabolites. Compared with the bud stage, the metabolites changed more significantly during flowering. (3) Multiple comparison analysis showed that the content of 26 flavonoids differed significantly (P < 0.05) in flowers at different phenological stages. Afzelin, kaempferitrin, quercitrin, hesperidin taxifolin and apigenin 7-O-glucuronide were higher at the flower opening stage. Final bud stage had higher concentrations of proanthocyanidin A2 and three proanthocyanidin trimers. 【Conclusion】 The development status could be an important factor in determining the content of secondary metabolites in the flowers of T. miqueliana. The signature compound at the final bud stage was proanthocyanidin A2, and at full bloom stage is apigenin-7-O-glucuronide, both of them were valuable for harvesting.

    参考文献
    相似文献
    引证文献
引用本文

原晓,王欢利,王奇志,等.南京椴花化学成分鉴定及不同发育时期黄酮类化合物差异分析[J].西北植物学报,2024,44(2):270-279

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2023-07-24
  • 最后修改日期:2023-09-25
  • 录用日期:2023-10-07
  • 在线发布日期: 2024-01-19
  • 出版日期:

微信公众号二维码

手机版网站二维码