仲恺农业工程学院 农业与生物学院 广东 广州 510631
Natural Science Foundation of Guangdong Province, (2021A1515010517, 2022A1515010798); Innovation and Entrepreneurship Training Program for College Students, (S202211347059)
【目的】核糖体蛋白（ribosomal protein，RP）是参与蛋白质合成及基因表达调控的一种重要因子，在植物生长发育和胁迫响应过程中具有重要的作用。研究在水稻中克隆了1个核糖体蛋白家族基因OsRPL36A，并对其生物学功能进行初步研究，为后续OsRPL36A基因功能研究提供理论依据和研究方向。【方法】利用生物信息学技术分析OsRPL36A基因结构、顺式作用元件和演化过程；同时利用实时荧光定量PCR（quantitative real-time PCR，qRT-PCR）技术分析OsRPL36A的组织表达特异性、节律表达模式、及其对不同激素和非生物胁迫的响应情况。【结果】（1）OsRPL36A的编码区全长为297 bp，共编码98个氨基酸，属于核糖体蛋白L36超基因家族。（2）OsRPL36A的启动子区包含3个节律表达相关元件、10个光响应元件、14个激素响应元件和27个环境胁迫响应元件。（3）OsRPL36A在叶片中的表达量相对高于其他组织；具有典型的节律表达模式；且受IAA、高温、低温和渗透胁迫等诱导表达。【结论】OsRPL36A在叶中高表达，具有典型节律表达模式，对IAA显著响应，可能参与热激、低温、盐害和渗透胁迫响应。
[Objective] As an important regulator involved in protein synthesis and gene expression regulation, ribosomal protein is crucial for plant growth and development and stress response. In this study, a ribosomal protein family gene OsRPL36A was cloned from rice, and preliminary research was performed on its biological function, which provided a theoretical basis and research direction for the subsequent study of OsRPL36A gene function. [Methods] Bioinformatics techniques were used to analysis the gene structure, cis-regulatory elements and evolution process of OsRPL36A. Meanwhile, the gene expression level of OsRPL36A in different tissues, under diurnal rhythm, different hormones and abiotic stresses were analyzed by quantitative real-time PCR (qRT-PCR). [Results] (1) The gene OsRPL36A encoded 98 amino acids in total with a 297 bp coding region, which belonged to the ribosomal protein L36 supergene family and was named as OsRPL36A. (2) The promoter region of OsRPL36A contained 3 rhythmic expression related elements, 10 light-responsive elements, 14 hormone-responsive elements, and 27 environmental stresses response elements. (3) The expression level of OsRPL36A in leaves was relatively higher than that in other tissues and presented a typical rhythmic expression pattern; the expression level of OsRPL36A was also induced by IAA, high temperature, low temperature and osmotic stress and so on. [Conclusion] OsRPL36A was highly expressed in leaves and presented a typical rhythmic expression pattern. OsRPL36A significantly responded to IAA and might be involved in response to heat shock, low temperature, salt stress, and osmotic stress.