【Objective】 As for the aspect of species representativeness and diversity, the article aims to provide a scientific evidence for plant diversity conservation, plant resource utilization in Qinghai area of Kunlun Mountains National Park. 【Methods】 Based on field surveys, historical data and literature, sorted out the list of seed plants in the assessment area, and analyzed the composition, dominant taxa, geographic component and endangered species of seed flora were assessed in the area. 【Results】 The results showed that: (1) There was a total of 46 families, 146 genera and 383 species of seed plants in Qinghai area of the Kunlun Mountain National Park, including three families, three genera and five species of gymnosperms, and 43 families, 143 genera and 379 species of angiosperms; the four families including Amaranthaceae, Asteraceae, Fabaceae and Poaceae occupy an important position in the seed flora of the assessed area, and monospecie and oligospecie constitute the main part of the family. (2) The family and genus dominances of seed plants were obvious in this area with eight dominant families and 36 dominant genera, six large genera had more than 10 species including Astragalus, Oxytropis, Artemisia, Saussurea, Poa and Carex (3) The areal-type of the families in this seed flora was mainly cosmopolitan, meanwhile it showed a temperate nature, and the areal-type of genera was mainly temperate distribution with a close connection to the Laurasia. (4) There were four species of national Grade Ⅱ protected wild plants, eight species of Qinghai Province key protected plants, and six species listed in this area. 【Conclusion】 As a whole, the seed flora in Qinghai area of the Kunlun Mountains National Park was remarkably characterized by temperate nature and clear transitional property with low plant species richness, less endemic family, genus and species.