9个山茶品种对高温胁迫的光合生理响应及其耐热性综合评价
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1.上海植物园 上海城市植物资源开发应用工程技术研究中心;2.杭州植物园(杭州西湖园林科学研究院)

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国家自然科学基金项目(面上项目,重点项目,重大项目)(31960327);上海市绿化和市容管理局科技攻关项目(G210304)


Photosynthetic Physiological Response of 9 Camellia Cultivars under High Temperature Stress and Comprehensive Evaluation of Their Heat Tolerance
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National Natural Science Foundation Project(31960327);Science and technology project of Shanghai Landscaping and City Appearance Administrative Bureau(G210304);

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    摘要:

    【目的】为山茶属植物耐热性的鉴定、耐热种质发掘和新品种选育提供理论依据。【方法】本研究以单体红山茶和国外束花茶花品种‘串花瀑布’作为对照材料,以7个自育束花茶花新品种为试材,经过高温短期(5h)和长期(7d)处理后,比较形态、生理和光合特性等指标的变化,并采用相关性分析、主成分分析对其耐热性进行综合评价。【结果】(1)‘垂枝粉玉’和‘串花瀑布’在高温胁迫下,叶绿素和类胡萝卜素含量、叶绿素a/b、Pn和Gs均上升,Fv/Fm、Fv/Fo和ETR维持在较高水平,且在高温长期胁迫7d后均未出现热害。‘金叶粉玉’、‘上植华章’和‘玫瑰春’在高温胁迫下Pn和Gs均显著降低,而Ci增高,其中‘金叶粉玉’和‘上植华章’的Fv/Fm、Fv/Fo和ETR均大幅降低,在高温长期胁迫7d后,这3个品种出现了较严重的热害或死亡。(2)通过主成分分析将15个单项指标换算成4个独立的综合指标,累计贡献率可达90%,高温胁迫5h时以叶绿素含量、类胡萝卜素含量、叶黄素含量这3个指标的综合权重值较高,高温胁迫7d时以叶绿素a/b、Fv/Fm、qP、qN这4个指标的综合权重值较高。其中高温胁迫7d时综合评价值D的大小依次为单体红山茶>‘串花瀑布’>‘垂枝粉玉’>‘上植月光曲’>‘上植欢乐颂’>‘玫玉’>‘玫瑰春’>‘上植华章’>‘金叶粉玉’。【结论】将9个品种划分出3个耐热等级,‘垂枝粉玉’和‘串花瀑布’为耐热品种,‘上植月光曲’、‘上植欢乐颂’和‘玫玉’为中等耐热品种,‘玫瑰春’、‘上植华章’和‘金叶粉玉’3个品种的耐热性最弱,而单体红山茶的耐热性有待进一步验证。其中叶绿素含量、类胡萝卜素含量、叶黄素含量这3个指标可作为分析山茶短期高温胁迫的关键指标;叶绿素a/b、Fv/Fm、qP、qN这4个指标可作为分析山茶耐受长期高温胁迫能力强弱的关键指标。

    Abstract:

    【Aim】To provide theoretical basis for the heat-tolerance identification of genus Camellia, the discovery of heat-tolerance germplasm and the breeding of new cultivars.【Method】In this study, C. uraku and ‘Wirlinga Cascade’, a foreign cultivar were used as reference materials,7 new cultivars of cluster-flowering camellia were selected as test materials,after short-term (5h) and long-term (7d) high-temperature treatment, morphological, physiological and photosynthetic characteristics were observed. The heat tolerance was evaluated by correlation analysis and principal component analysis.【Results】(1) The contents of chlorophyll and carotenoid, chlorophyll a/b, Pn and Gs all increased, Fv/Fm, Fv/Fo and ETR remained at a high level, and also ‘Chuizhi Fenyu’ and ‘Wirlinga Cascade’ did not appear heat damage at 7 days of high temperature stress.Pn and Gs of 'Jinye Fenyu', 'Shangzhi Huazhang' and 'Meigui Chun' all decreased significantly under high temperature stress, while Ci increased. Among them, the Fv/Fm, Fv/Fo and ETR of 'Jinye Fenyu' and 'Shangzhi Huazhang' were significantly reduced, and after 7 days of long-term high temperature stress, these 3 cultivars showed serious heat damage or death. (2)15 individual indicators were converted into 4 independent comprehensive indicators with cumulative contribution rate of 90% through principal component analysis.The comprehensive weight values of chlorophyll content, carotenoid content and lutein content were higher at 5h under high temperature stress, and the comprehensive weight values of chlorophyll a/b, Fv/Fm, qP and qN were higher at 7d under high temperature stress. Under high temperature stress for 7 days, from the largest to the smallest of comprehensive evaluation value D was as follows: Camellia uraku >‘Wirlinga Cascade’>‘Chuizhi Fenyu’>‘Shangzhi Yueguangqu’>‘Shangzhi Huanlesong’> ‘Sweet Gem’>‘Meigui Chun’> 'Shangzhi Huazhang' >‘Jinye Fenyu’.【Conclusions】According to the comprehensive evaluation value D, the 9 cultivars were divided into 3 heat tolerance grades, 'Chuizhi Fenyu' and ‘Wirlinga Cascade’, were heat tolerance cultivars, 'Shangzhi Yueguangqu', 'Shangzhi Huanlesong' and 'Sweet Gem' were medium heat tolerance cultivars, and 'Meigui Chun', 'Shangzhi Huazhang' and 'Jinye Fenyu' had the weakest heat tolerance,the heat resistance of C. uraku needs to be further verified.Chlorophyll content, carotenoid content, xanthophyll content can be used as the key indexes to analyze the short-term high temperature stress of Camellia. Chlorophyll a/b,Fv/Fm,qP, qN,these 4 indexes can be used as the key indexes to analyze the long-term high temperature stress of Camellia.

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郭卫珍,宋垚,章丹峰,等.9个山茶品种对高温胁迫的光合生理响应及其耐热性综合评价[J].西北植物学报,2024,44(4):539-550

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  • 收稿日期:2023-09-20
  • 最后修改日期:2024-01-11
  • 录用日期:2024-01-13
  • 在线发布日期: 2024-04-03
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