水稻OsMGD2和OsMGD3基因的功能鉴定及对烟草耐低磷胁迫的影响
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1.西北农林科技大学 资源环境学院;2.商洛学院,陕西商洛;3.西北农林科技大学农学院

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陕西省自然科学基础研究计划2021JM-604


Functional Identification of OsMGD2 and OsMGD3 Gene in Rice and their Effects in Response to Phosphorus Deficiency in Tobacco
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Natural Science Basic Research Program of Shaanxi Province 2021JM-604

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    摘要:

    【目的】单半乳糖甘油二酯合酶(MGD)是合成单半乳糖甘油二酯(MGDG)的关键酶,在植物响应低磷胁迫过程中起着重要作用。为系统了解水稻OsMGD2和OsMGD3基因在低磷胁迫响应中的作用和功能。【方法】采用盆栽试验分析正常和低磷条件下野生型(SR1)和过表达OsMGD2和OsMGD3转基因烟草的生理响应和脂质组分的变化。【结果】无论在正常还是低磷条件下,转基因烟草与野生型的磷含量无显著差异,然而转基因烟草的生物量、叶绿素含量和光合能力均显著高于野生型。转基因烟草在低磷胁迫下的磷脂(PL)含量、双半乳糖甘油二酯(DGDG)含量、DGDG/MGDG比值和半乳糖脂(GL)/PL比值均显著高于野生型烟草,且OsMGD3转基因烟草的脂质含量和比值均高于OsMGD2转基因烟草。【结论】通过调控水稻OsMGD2/3基因表达,可提高植物低磷下的膜脂重塑能力,维持植物在低磷胁迫下的较高的光合和生长能力,增加植物的低磷耐受性。

    Abstract:

    【Objective】 Monogalactose diglyceride synthase (MGD) is a key enzyme in the synthesis of monogalactose diglyceride (MGDG) and plays an important role in plant response to low phosphorus tolerance. To systematically understand the role and function of OsMGD2 and OsMGD3 genes in response to phosphorus(P) deficiency. 【Methods】 A pot experiment was conducted to analysis physiological responses and lipid composition changes of wild-type (SR1) and transgenic tobacco under normal and P deficiency condition.【Results】 The was no difference in P content between transgenic and wild-type tobacco under normal and P deficiency conditions. However, the biomass, chlorophyll content, and photosynthetic electronic transfer of transgenic tobacco were significantly higher than wild type. The phospholipid (PL) content, digalactose diglyceride (DGDG) content, DGDG/MGDG ratio and galactosyllipid (GL)/PL ratio of transgenic tobacco were significantly higher than those of wild type tobacco under low phosphorus deficiency, and the lipid content and ratio of OsMGD3 transgenic tobacco were higher than OsMGD2 transgenic tobacco.【Conclusion】 Regulation of OsMGD2/3 gene expression in rice can improve the membrane lipid remodeling ability of plants under phosphorus deficiency, maintain the higher photosynthetic and growth capacity of plants under phosphorus deficiency, and increase the plant tolerance to low phosphorus stress.

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席 源,张梅娟,李莎莎,等.水稻OsMGD2和OsMGD3基因的功能鉴定及对烟草耐低磷胁迫的影响[J].西北植物学报,2024,44(5):716-728

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历史
  • 收稿日期:2023-11-14
  • 最后修改日期:2023-12-20
  • 录用日期:2024-01-25
  • 在线发布日期: 2024-04-25
  • 出版日期: 2024-05-25