[Objective] The Gretchen Hagen 3 (GH3) gene family is a class of auxin early response genes, which play an important role in plant growth and development and stress resistance. The objective of this study was to investigate the function and effect of GH3 gene in kidney bean. [Methods] The whole genome of kidney bean GH3 gene family was identified by bioinformatics and the expression of PvGH3 gene was detected by transcriptome data (RNA-seq) and fluorescent quantitative PCR (RT-qPCR) under different stress conditions. [Results] A total of 19 gene family members were identified in kidney bean, of which 17 genes were unevenly distributed on 8 chromosomes, and the other 2 genes could not be accurately located on scafford. Phylogenetic analysis divided the PvGH3 gene family into three subfamilies, and the members of each subfamily had similar gene structure and conserved motifs. The collinearity analysis showed that there were 3 para-homologous gene pairs between PvGH3 family members, and 15 direct homologous gene pairs between PvGHs and Arabidopsis GH3.Cis-element analysis showed that PvGH3 gene family contains cis-elements related to light, hormone, stress and growth and development. Tissue expression analysis showed that PvGH3 gene family was highly expressed in flowers, roots, buds and stems. qRT-PCR analysis showed that 19 PvGH3 were up-regulated in response to drought (6% PEG), salt (100 mmol/L NaCl) and low temperature (4 ℃) stress. PvGH3.19 was significantly responsive to drought stress, PvGH3.17 was responsive to salt stress. PvGH3.11 responds to low temperature stress. [Conclusion] In this study, 19 PvGH3 genes were identified in kidney bean. Their physicochemical properties, chromosomal location, collinearity, evolutionary relationship, conserved motif, cis-element and tissue expression were analyzed, and their functions and effects were further demonstrated by qRT-PCR. This study provided a theoretical basis for the analysis of resistance function of PvGH3.