过表达榨菜BjuJAZ8增强拟南芥对盐胁迫的敏感性
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长江师范学院 现代农业与生物工程学院

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重庆市教育委员会科学技术研究项目(KJQN202201407);重庆市教育委员会科学技术研究重点项目(KJZD-K202301407);重庆市高校创新研究群体资助计划(CXQT21029)


Overexpression of BjuJAZ8 in tuber mustard enhanced the sensitivity of Arabidopsis thaliana to salt stress
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    【目的】榨菜具有较高的经济价值,探讨榨菜中茉莉酸信号通路基因JAZ8在植物响应盐胁迫的功能可为榨菜抗逆品种培育提供基因资源。【方法】采用生物信息学方法分析榨菜JAZ8的基因结构、启动子顺式作用元件以及所编码蛋白的理化性质;采用分子生物学技术克隆榨菜JAZ8基因并构建其过表达载体;采用转基因技术在模式植物拟南芥中过表达榨菜榨菜JAZ8基因并分析该基因在拟南芥响应盐胁迫过程中的具体功能。【结果】榨菜JAZ8基因蛋白编码序列全长396 bp,编码131个氨基酸,含有TIFY和CCT-2结构域;该基因启动子含有响应脱落酸、水杨酸及参与防御和应激反应的顺式作用元件;转基因植株表型分析结果表明,过表达榨菜JAZ8的拟南芥植株在萌发和转绿阶段表现为对盐胁迫更加敏感的表型。【结论】过表达榨菜JAZ8增强拟南芥对盐胁迫的敏感性。

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    [Objective] Tuber mustard has high economic value. Investigating the function of jasmonic acid signaling pathway gene JAZ8 in plant response to salt stress can provide genetic resources for breeding resistant varieties of tuber mustard. [Methods] The gene structure, promoter cis-acting elements and the physical and chemical properties of JAZ8 were analyzed by bioinformatics method. JAZ8 gene was cloned by molecular biology and overexpression vector was constructed. The JAZ8 gene of tuber mustard was overexpressed in the model plant Arabidopsis Thaliana by transgenic technology and the specific function of JAZ8 gene in Arabidopsis thaliana in response to salt stress was analyzed. [Results] The JAZ8 gene coding sequence was 396 bp, encoding 131 amino acids, containing TIFY and CCT-2 domains. The gene promoter contains cis-acting elements that respond to abscisic acid, salicylic acid and participate in defense and stress response. The phenotypic analysis of transgenic plants showed that Arabidopsis plants overexpressing BjuJAZ8 showed a more sensitive phenotype to salt stress during germination and greening. [Conclusion] Overexpression of JAZ8 in tuber mustard enhanced the sensitivity of Arabidopsis to salt stress.

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蔡兆明,程春红,余 红,等.过表达榨菜BjuJAZ8增强拟南芥对盐胁迫的敏感性[J].西北植物学报,2024,44(11):1743-1751

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  • 收稿日期:2024-05-11
  • 最后修改日期:2024-06-21
  • 录用日期:2024-06-21
  • 在线发布日期: 2024-09-24
  • 出版日期: 2024-11-04
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